| Identification of a novel conserved motif in the STAT family that is required for tyrosine phosphorylation. | |
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MedLine Citation:
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PMID: 14722125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rapid transcriptional activation of cellular genes by either type 1 interferons (IFNalpha/beta) or type 2 interferon (IFNgamma) is responsible for many of the pleiotropic effects of these cytokines, including their antiviral, antigrowth, and immunomodulatory activities. Interferon-stimulated gene expression is mediated by transcription factors termed Stats, which upon being tyrosine-phosphorylated, translocate to the nucleus and bind enhancers of interferon-activated genes. We have recently characterized a new Jurkat cell variant, named H123, where IFNalpha stimulates programmed cell death. H123 clones that are resistant to the apoptotic actions of IFNalpha have been selected. One of these clones (Clone 8) is defective in its responses to IFNalpha with regard to activation of genes that require tyrosine phosphorylation of Stat2. Stimulation of Clone 8 cells with IFNalpha induces normal tyrosine phosphorylation of Stat1 and Stat3. Sequencing of Stat2 RNA reveals a substitution of proline 630 located within the Src homology 2 domain of Stat2 to leucine (P630L). Pro-630 and its adjacent amino acids are conserved in all Stat family members but are absent in other proteins that contain Src homology 2 domains. Expression of Stat2 P630L in cells inhibits IFNalpha-stimulated gene expression. These results not only define a critical motif in Stat2 required for its transcriptional activity, but they also provide evidence that resistance to type one IFNs can be mediated by mutations in Stat2 as well as those previously described for Stat1. |
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Authors:
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Ana M Gamero; Shuji Sakamoto; Javier Montenegro; Andrew C Larner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2004-01-13 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 279 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-22 Completed Date: 2004-05-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 12379-85 Citation Subset: IM |
Affiliation:
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Department of Immunology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus Amino Acid Motifs Amino Acid Sequence Apoptosis Base Sequence Blotting, Western Conserved Sequence DNA-Binding Proteins / metabolism* Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Flow Cytometry Gene Expression Regulation Humans Interferon-alpha / metabolism Interferon-beta / metabolism Interferon-gamma / metabolism Jurkat Cells Leucine / chemistry Luciferases / metabolism Microscopy, Fluorescence Molecular Sequence Data Phosphorylation Precipitin Tests Proline / chemistry Protein Structure, Tertiary RNA / metabolism Reverse Transcriptase Polymerase Chain Reaction Ribonucleases / chemistry STAT1 Transcription Factor STAT2 Transcription Factor STAT3 Transcription Factor Sequence Homology, Amino Acid Trans-Activators / metabolism* Transcription, Genetic Transcriptional Activation* Transfection Tyrosine / chemistry*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA 77366/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Interferon-alpha; 0/STAT1 Transcription Factor; 0/STAT1 protein, human; 0/STAT2 Transcription Factor; 0/STAT2 protein, human; 0/STAT3 Transcription Factor; 0/STAT3 protein, human; 0/Trans-Activators; 147-85-3/Proline; 55520-40-6/Tyrosine; 61-90-5/Leucine; 63231-63-0/RNA; 77238-31-4/Interferon-beta; 82115-62-6/Interferon-gamma; EC 1.13.12.-/Luciferases; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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