| Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. | |
| | |
MedLine Citation:
|
PMID: 12527899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G(2)/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells. |
| | |
Authors:
|
Ann-Ping Tsou; Chu-Wen Yang; Chi-Ying F Huang; Ricky Chang-Tze Yu; Yuan-Chii G Lee; Cha-Wei Chang; Bo-Rue Chen; Yu-Fang Chung; Ming-Ji Fann; Chin-Wen Chi; Jen-Hwey Chiu; Chen-Kung Chou |
Related Documents
:
|
3697979 - Double elution analysis of cross-link formation and repair in cycling and noncycling 9l... 22484909 - Overactivation of ras signaling pathway in cd133+ mpnst cells. 16771839 - Two cell-cycle regulated set-domain proteins interact with proliferating cell nuclear a... 20170199 - Characterization of cell cycle specific protein interaction networks of the yeast 26s p... 12934019 - Expression deconvolution: a reinterpretation of dna microarray data reveals dynamic cha... 20870879 - Reorganization of the growth pattern of schizosaccharomyces pombe in invasive filament ... 8380669 - Simian foamy virus type 3 (sfv-3) in latently infected vero cells: reactivation by deme... 2113339 - Immunohistochemical investigations in epidermal merkel cells--a common phenotype with e... 2701479 - Use of primary cell culture to study regulation of airway surface epithelial mucus secr... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Oncogene Volume: 22 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2003 Jan |
Date Detail:
|
Created Date: 2003-01-15 Completed Date: 2003-02-04 Revised Date: 2011-10-13 |
Medline Journal Info:
|
Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
|
Languages: eng Pagination: 298-307 Citation Subset: IM |
Affiliation:
|
Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, Taiwan. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AB076695; AB076696 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Carcinoma, Hepatocellular / genetics* Cell Cycle / genetics* Cloning, Molecular Databases, Nucleic Acid Expressed Sequence Tags Liver / embryology, physiology Liver Neoplasms / genetics* Liver Regeneration / genetics Mice Mice, Inbred BALB C Mitosis Mitotic Spindle Apparatus / genetics Molecular Sequence Data Neoplasm Proteins / genetics*, metabolism* Organ Specificity Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
|
0/DLGAP5 protein, human; 0/Neoplasm Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DEDD and DEDD2 associate with caspase-8/10 and signal cell death.
Next Document: Distinct changes in gene expression induced by A-Myb, B-Myb and c-Myb proteins.