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Identification of a novel adhesin involved in acid-induced adhesion of enterohemorrhagic Escherichia coli O157:H7.
MedLine Citation:
PMID:  22767547     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Enterohemorrhagic Escherichia coli (EHEC) survives exposure to acute acid stress during gastric passage and progresses to colonize the large intestine. We previously reported that acid stress significantly increases host-adhesion of EHEC O157:H7 and is associated with a coincident upregulation of the expression of a putative adhesin gene, yadK. Further gene expression analysis now confirms that yadK is minimally transcribed under unstressed conditions and is significantly upregulated under acid stress. Immunoblotting with an anti-YadK polyclonal antiserum demonstrates that YadK protein is also upregulated after acid stress. Disruption of yadK results in loss of the acid-induced adhesion increase seen for wild type EHEC to human epithelial cells in vitro and complementation in trans fully restores the acid-induced adhesion phenotype to wild-type level. Significantly, no difference is observed in adhesion of the unstressed yadK mutant relative to wild type, indicating that YadK does not play a role in adhesion of unstressed EHEC. Anti-YadK antiserum inhibits the acid-induced adhesion enhancement of EHEC but has no effect on adhesion of unstressed EHEC. There is no significant difference in the viability of either unstressed or the acid-stressed yadK mutant relative to the similarly treated wild type, suggesting that yadK is not involved in acid tolerance. These results provide persuasive evidence that YadK plays a significant role in the adhesion of acid-stressed EHEC to epithelial cells, and support a role for acid stress as a factor which may regulate bacteria-host attachment and lead to increased EHEC colonization and virulence.
Authors:
Frances Chingcuanco; Yijing Yu; Julianne V Kus; Lynn Que; Tracy Lackraj; Céline M Levesque; Debora E Barnett Foster
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-5
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemistry and Biology, Ryerson University;
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