| Identification of a novel adhesin involved in acid-induced adhesion of enterohemorrhagic Escherichia coli O157:H7. | |
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MedLine Citation:
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PMID: 22767547 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Enterohemorrhagic Escherichia coli (EHEC) survives exposure to acute acid stress during gastric passage and progresses to colonize the large intestine. We previously reported that acid stress significantly increases host-adhesion of EHEC O157:H7 and is associated with a coincident upregulation of the expression of a putative adhesin gene, yadK. Further gene expression analysis now confirms that yadK is minimally transcribed under unstressed conditions and is significantly upregulated under acid stress. Immunoblotting with an anti-YadK polyclonal antiserum demonstrates that YadK protein is also upregulated after acid stress. Disruption of yadK results in loss of the acid-induced adhesion increase seen for wild type EHEC to human epithelial cells in vitro and complementation in trans fully restores the acid-induced adhesion phenotype to wild-type level. Significantly, no difference is observed in adhesion of the unstressed yadK mutant relative to wild type, indicating that YadK does not play a role in adhesion of unstressed EHEC. Anti-YadK antiserum inhibits the acid-induced adhesion enhancement of EHEC but has no effect on adhesion of unstressed EHEC. There is no significant difference in the viability of either unstressed or the acid-stressed yadK mutant relative to the similarly treated wild type, suggesting that yadK is not involved in acid tolerance. These results provide persuasive evidence that YadK plays a significant role in the adhesion of acid-stressed EHEC to epithelial cells, and support a role for acid stress as a factor which may regulate bacteria-host attachment and lead to increased EHEC colonization and virulence. |
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Authors:
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Frances Chingcuanco; Yijing Yu; Julianne V Kus; Lynn Que; Tracy Lackraj; Céline M Levesque; Debora E Barnett Foster |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-5 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: - ISSN: 1465-2080 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Chemistry and Biology, Ryerson University; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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