Document Detail


Identification of novel DNA fragments and partial sequence of a genomic island specific of Brucella pinnipedialis.
MedLine Citation:
PMID:  18514443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since the 1990s, Brucella strains have been isolated from a wide variety of marine mammals and were recently recognized as two different species, i.e. Brucella pinnipedialis for pinniped isolates and Brucella ceti for cetacean isolates. The aim of this study was to identify specific DNA fragments of marine mammal Brucella strains using a previously described infrequent restriction site-PCR (IRS-PCR) method but with three new couples of restriction enzymes applied on a larger panel of marine mammal Brucella isolates (n=74) and one human isolate from New Zealand likely from marine mammal origin. This study revealed five DNA fragments specific of Brucella strains isolated from marine mammals. Among them two new DNA fragments were specific of B. pinnipedialis but were not detected in hooded seal isolates. DNA fragment I identified in the previous IRS-PCR study and fragment VI of this study were located on a cloned and sequenced 6kb SacI fragment. Its nucleotide sequence revealed that it is likely part of a putative genomic island. Sequence analysis showed that it carries four ORFs coding for putative metabolic functions. Although hooded seal isolates are classified within B. pinnipedialis it was shown in this study that they do not carry this genomic island and this raises the question about their evolutionary history within B. pinnipedialis.
Authors:
Marianne Maquart; Yann Fardini; Michel S Zygmunt; Axel Cloeckaert
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Publication Detail:
Type:  Journal Article     Date:  2008-06-02
Journal Detail:
Title:  Veterinary microbiology     Volume:  132     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2009-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  181-9     Citation Subset:  IM    
Affiliation:
Institut National de la Recherche Agronomique, UR1282, Infectiologie Animale et Santé Publique, Nouzilly, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brucella / classification*,  genetics*
Cetacea / microbiology
DNA, Bacterial / genetics,  isolation & purification*
Evolution, Molecular
Genomic Islands / genetics*
Pinnipedia / microbiology
Polymerase Chain Reaction / veterinary
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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