Document Detail

Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro.
MedLine Citation:
PMID:  21820435     Owner:  NLM     Status:  MEDLINE    
While there is evidence of nitric oxide (NO)-dependent signalling via the second messenger cyclic guanosine 3',5'-monophosphate (cGMP) in plants, guanylate cyclases (GCs), enzymes that catalyse the formation of cGMP from guanosine 5'-triphosphate (GTP) have until recently remained elusive and none of the candidates identified to-date are NO-dependent. Using both a GC and heme-binding domain specific (H-NOX) search motif, we have identified an Arabidopsis flavin monooxygenase (At1g62580) and shown electrochemically that it binds NO, has a higher affinity for NO than for O(2) and that this molecule can generate cGMP from GTP in vitro in an NO-dependent manner.
Takalani Mulaudzi; Ndiko Ludidi; Oziniel Ruzvidzo; Monique Morse; Nicolette Hendricks; Emmanuel Iwuoha; Chris Gehring
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-31
Journal Detail:
Title:  FEBS letters     Volume:  585     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-29     Completed Date:  2011-10-27     Revised Date:  2014-01-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2693-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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MeSH Terms
Amino Acid Sequence
Arabidopsis / enzymology,  genetics,  metabolism*
Arabidopsis Proteins / chemistry,  genetics,  metabolism*
Guanylate Cyclase / chemistry,  metabolism*
Mixed Function Oxygenases / genetics,  metabolism*
Molecular Sequence Data
Nitric Oxide / metabolism*
Oxygen / metabolism
Reg. No./Substance:
0/Arabidopsis Proteins; 31C4KY9ESH/Nitric Oxide; EC 1.-/Mixed Function Oxygenases; EC 1.-/NOGC1 protein, Arabidopsis; EC Cyclase; S88TT14065/Oxygen

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