| Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphism. | |
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MedLine Citation:
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PMID: 12039784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper presents the finding of the possible cause of the high false-positive rate in acid-fast staining in histological examinations. Using acid-fast staining, culture, and PCR, acid-fast bacilli were detected in 83.7% of 49 hospital tap water samples and nontuberculous mycobacteria (NTM) were detected in 20.4% of the same 49 samples. The 10 NTM isolates were also identified to the species level using PCR-restriction fragment length polymorphism. Our findings indicate that NTM in hospital tap water are the possible cause of false positives in acid-fast staining and of nosocomial infection in immunocompromised patients. |
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Authors:
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Chiao-Tang Chang; Ling-Yu Wang; Chen-Yi Liao; Shiao-Ping Huang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 68 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-05-31 Completed Date: 2002-07-22 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3159-61 Citation Subset: IM |
Affiliation:
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Department of Medical Research, Yuan's General Hospital, Kaohsiung, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cross Infection
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microbiology Immunocompromised Host / immunology Mycobacterium / genetics, isolation & purification* Mycobacterium Infections / microbiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Water Microbiology* |
| Comments/Corrections | |
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