Document Detail

Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphism.
MedLine Citation:
PMID:  12039784     Owner:  NLM     Status:  MEDLINE    
This paper presents the finding of the possible cause of the high false-positive rate in acid-fast staining in histological examinations. Using acid-fast staining, culture, and PCR, acid-fast bacilli were detected in 83.7% of 49 hospital tap water samples and nontuberculous mycobacteria (NTM) were detected in 20.4% of the same 49 samples. The 10 NTM isolates were also identified to the species level using PCR-restriction fragment length polymorphism. Our findings indicate that NTM in hospital tap water are the possible cause of false positives in acid-fast staining and of nosocomial infection in immunocompromised patients.
Chiao-Tang Chang; Ling-Yu Wang; Chen-Yi Liao; Shiao-Ping Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-31     Completed Date:  2002-07-22     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3159-61     Citation Subset:  IM    
Department of Medical Research, Yuan's General Hospital, Kaohsiung, Taiwan, Republic of China.
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MeSH Terms
Cross Infection / microbiology
Immunocompromised Host / immunology
Mycobacterium / genetics,  isolation & purification*
Mycobacterium Infections / microbiology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Water Microbiology*

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