| Identification of a new vascular smooth muscle contracting polypeptide in Phoneutria nigriventer spider venom. | |
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MedLine Citation:
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PMID: 8216354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fractionation of Phoneutria nigriventer spider venom by gel filtration (Sephadex G-10-120) followed by ion-exchange chromatography (microgranular CM-cellulose-52) resulted in sixteen fractions (CI to CXVI) from which CVII+VIII, CIX and CX+XI caused dose-dependent and short-lived contractions of both arterial and venous rabbit vessels. Fraction CX+XI was further purified by a reverse phase HPLC, and a contractile polypeptide (PNV2) was isolated. The amino terminal sequence of PNV2 (LAKRADICQPGKTSQRACET) indicated that it represents a pure polypeptide consisting of a single chain. Furthermore, the amino acid analysis of PNV2 revealed the presence of four disulfide bridges, a high content in Lys (14%), Glx (11%), and the absence of His. The global amino acid composition showed that this polypeptide is composed of 102 residues (Trp not included) with a calculated molecular weight of 12,114. Whether this peptide is responsible for the vascular alterations observed in Phoneutria envenomation, such as lung edema and priapism, remains to be further investigated. |
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Authors:
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A C Bento; J C Novello; S Marangoni; E Antunes; J R Giglio; B Oliveira; G de Nucci |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical pharmacology Volume: 46 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-11-01 Completed Date: 1993-11-01 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1092-5 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Campinas UNICAMP, SP, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Male Molecular Sequence Data Muscle Contraction / drug effects Muscle, Smooth, Vascular / drug effects* Neuropeptides / chemistry, isolation & purification*, pharmacology Peptides / chemistry, isolation & purification*, pharmacology Rabbits Spider Venoms / chemistry*, isolation & purification*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptides; 0/PNV2 peptide; 0/Peptides; 0/Spider Venoms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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