Document Detail


Identification of a new interferon-alpha-inducible gene (p27) on human chromosome 14q32 and its expression in breast carcinoma.
MedLine Citation:
PMID:  8358738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new complementary DNA, p27, has been cloned and sequenced from estradiol-treated MCF7 human breast carcinoma cells. It encodes a putative highly hydrophobic protein of 122 amino acids which has a 33% overall sequence similarity to the product of the 6-16 gene (R. L. Friedman, S. P. Manly, M. McMahon, I. M. Kerr, and G. R. Stark, Cell, 38: 745-755, 1984), which is transcriptionally induced by interferons of the alpha/beta type. We demonstrate here that the p27 gene, which is located in band q32 of human chromosome 14, is also induced by interferon-alpha in human cell lines of different origin and that expression is independent of the presence of estradiol receptor in the cells. High levels of p27 RNA were found in vivo in approximately 50% of primary human breast carcinomas (21 were tested by Northern blotting). In situ hybridization to some of the p27-overexpressing tumors showed that the p27 RNA is localized in cancer cells and sometimes also in fibroblastic cells of tumor stroma. p27 RNA levels in the tumors did not correlate with the presence of estrogen receptor or with the expression of the estrogen-induced pS2 gene. Further studies are now necessary to elucidate the cause of p27 gene overexpression in breast carcinoma and in particular to determine whether it corresponds to chromosomal rearrangements in the 14q32 region and/or to induction by interferons of the alpha/beta type.
Authors:
U B Rasmussen; C Wolf; M G Mattei; M P Chenard; J P Bellocq; P Chambon; M C Rio; P Basset
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  53     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-09-24     Completed Date:  1993-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4096-101     Citation Subset:  IM    
Affiliation:
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, INSERM-Institut de Chimie Biologique-Faculté de Médecine, Strasbourg, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S55623;  S55625;  S55628;  S55677;  S55678;  S55680;  S55739;  S65854;  X65706;  X67325
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Breast Neoplasms / genetics*
Chromosome Mapping*
Chromosomes, Human, Pair 14*
DNA, Neoplasm / chemistry,  genetics*
Gene Expression / drug effects*
Humans
In Situ Hybridization
Interferon-alpha / pharmacology*
Molecular Sequence Data
Nucleic Acid Hybridization
RNA, Messenger / analysis
RNA, Neoplasm / analysis
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Interferon-alpha; 0/RNA, Messenger; 0/RNA, Neoplasm

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