| Identification of a new genetic determinant for cell aggregation associated with lactose plasmid transfer in Lactococcus lactis. | |
| | |
MedLine Citation:
|
PMID: 1903626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Derivatives of the lactose miniplasmid pMG820 were constructed in which a staphylococcal erm gene was inserted and in which this was accompanied by subsequent deletion of the lactose genes. The resulting plasmids were thus marked with both erythromycin resistance and lactose utilization genes in pF1132 or solely erythromycin resistance in pF1133. These plasmids retained the normal conjugation properties characteristic of lactose plasmid pLP712, including the generation by intermolecular rearrangement of high-frequency-transfer Clu+ derivatives which exhibited cell aggregation. The use of such Clu+ plasmids in a variety of mating experiments between different lactococcal strains and the observation of cell aggregation when particular mating mixtures were made led to the discovery of a new component of this conjugation system named Agg. A chromosomal gene agg was postulated to be present in some but not all strains of lactococci. High-frequency conjugation and cell aggregation thus depend on the presence of both Agg and Clu, although in a mating pair these components can be in the same or in separate strains. The Agg and Clu components may be analogous to the binding substance and aggregation substance that are involved in the hemolysin plasmid transfer system of Enterococcus faecalis, although control of their expression is different. |
| | |
Authors:
|
D van der Lelie; F Chavarri; G Venema; M J Gasson |
Related Documents
:
|
331756 - Mercury resistance and r plasmids in clinical isolates of kebsiella pneumoniae. 2992366 - Plasmid-borne or chromosomally mediated resistance by tn7 is the most common response t... 18606786 - Characterization of salmonella enterica serovar typhimurium conjugative plasmids transf... 2851686 - Rederivation of inbred strains of mice by means of embryo transfer. 19291016 - The value of 2d strain imaging during stress testing. 12376036 - Gatifloxacin phase iv surveillance trial (teqces study) utilizing 5000 primary care phy... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 57 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1991 Jan |
Date Detail:
|
Created Date: 1991-06-24 Completed Date: 1991-06-24 Revised Date: 2010-09-09 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 201-6 Citation Subset: IM |
Affiliation:
|
AFRC Institute of Food Research, Norwich Laboratory, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Conjugation, Genetic Genes, Bacterial* Lactococcus lactis / cytology, genetics* Lactose / genetics Plasmids* Species Specificity |
| Chemical | |
Reg. No./Substance:
|
63-42-3/Lactose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and properties of a (1,3)-beta-D-glucanase from Ruminococcus flavefaciens.
Next Document: Evaluation of hybridization characteristics of a cloned pRF106 probe for Listeria monocytogenes dete...