| Identification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays. | |
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MedLine Citation:
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PMID: 16416490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Finding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient alpha-glycosylation by the non-Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology. |
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Authors:
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Jürgen Seibel; Hendrik Hellmuth; Bernd Hofer; Anna-Maria Kicinska; Bodo Schmalbruch |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chembiochem : a European journal of chemical biology Volume: 7 ISSN: 1439-4227 ISO Abbreviation: Chembiochem Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-07 Completed Date: 2006-05-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: Germany |
Other Details:
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Languages: eng Pagination: 310-20 Citation Subset: IM |
Affiliation:
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Technical Chemistry, Department for Carbohydrate Technology, Technical University Braunschweig, Langer Kamp 5, 38106 Braunschweig, Germany. jseibel@tu-bs.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcohols
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chemistry,
metabolism Amino Acid Sequence Amino Acids / chemistry, metabolism Bacterial Proteins / chemistry, metabolism* Carbohydrate Conformation Carbohydrate Sequence Glycosylation Glycosyltransferases / chemistry, genetics, metabolism* Methylglucosides / biosynthesis Microarray Analysis / methods* Molecular Sequence Data Molecular Structure Oligosaccharides* / chemistry, metabolism Sequence Alignment Streptococcus oralis / enzymology Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Alcohols; 0/Amino Acids; 0/Bacterial Proteins; 0/Methylglucosides; 0/Oligosaccharides; 25360-07-0/methylglucoside; EC 2.4.-/Glycosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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