Document Detail


Identification of new DNA markers close to the myotonic dystrophy locus.
MedLine Citation:
PMID:  1672160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The most useful markers for the prenatal diagnosis of myotonic dystrophy (DM) are APOC2 and CKM, both of which map proximal to DM. In order to produce other markers useful for DM, we have screened genomic DNA libraries constructed from cell line 20XP3542-1-4, which contains 20 to 30 Mb of human material including APOC2 and CKM. Of 51 human clones identified, seven map to chromosome 17, four to chromosome 8, and nine to chromosome 19, and the remaining 31 were excluded form chromosome 19 but not localised further. Four of the clones from chromosome 19 map distal to CKM and two of these clones (D19S62 and D19S63) are closely linked to DM. Analysis of a family in which a crossover between CKM and DM has occurred shows that neither D19S62 nor D19S63 and DM have recombined, suggesting that D19S62 and D19S63 are either closer to or flanking DM in relation to CKM. Pulsed field gel analysis showed that CKM, D19S62, and D19S63 map to a region of at least 1500 kb.
Authors:
J D Brook; H G Harley; K V Walsh; S A Rundle; M J Siciliano; P S Harper; D J Shaw
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical genetics     Volume:  28     ISSN:  0022-2593     ISO Abbreviation:  J. Med. Genet.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-04-12     Completed Date:  1991-04-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985087R     Medline TA:  J Med Genet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  84-8     Citation Subset:  IM    
Affiliation:
Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
Chromosomes, Human, Pair 19*
Cloning, Molecular / methods
DNA / analysis
Electrophoresis, Agar Gel
Genetic Markers*
Humans
Linkage (Genetics)
Myotonic Dystrophy / genetics*
Nucleic Acid Hybridization
Pedigree
Polymorphism, Restriction Fragment Length*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Genetic Markers; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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