Document Detail


Identification of neuroblastoma stem cells by characterization of side population cells in the human neuroblastoma SK-N-SH cell line.
MedLine Citation:
PMID:  21129535     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
PURPOSE: The purpose of the study was to sort side population (SP) cells from the neuroblastoma SK-N-SH cell line and to systematically investigate whether this population has stem cell characteristics.
METHODS: Side population and non-SP cells were separated from the SK-N-SH cell line by flow cytometry, and their morphologic characteristics were analyzed by light and electron microscopy. We also used Western blotting to analyze marker proteins, Cell Counting Kit-8 assay for proliferative ability, series differentiation studies for differentiation properties, and Matrigel invasion study and tumorigenicity assay for malignant potential.
RESULTS: The SK-N-SH SP cells expressed high levels of stem cell markers, had high proliferative and malignant abilities, and had the capacity for rapid differentiation. The non-SP cells expressed differentiated cell marker proteins at high levels, had low proliferative and malignant abilities, and exhibited slow differentiation.
CONCLUSIONS: The SK-N-SH SP cells have cancer stem cell-like properties.
Authors:
Shiqin Qi; Jicui Zheng; Haitao Zhu; Lin Yang; Xianmin Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  45     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2305-11     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Surgery, Children's Hospital of Fudan University, Shanghai 201102, China.
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