| Identification of neoplastic cells in blood using the Sysmex XT-2000iV: a preliminary step in the diagnosis of canine leukemia. | |
| | |
MedLine Citation:
|
PMID: 20230575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Classification of leukemias requires specialized diagnostic techniques. Automated preliminary indicators of neoplastic cells in blood would expedite selection of appropriate tests. OBJECTIVE: The objective of this study was to assess the capacity of the Sysmex XT-2000iV hematology analyzer to identify neoplastic cells in canine blood samples. METHODS: Blood samples (n=160) were grouped into 5 categories: acute leukemia (n=30), chronic leukemia (n=15), neoplasia without blood involvement (n=41), non-neoplastic reactive conditions (n=31), and healthy dogs (n=43). WBC counts, WBC flags, scattergrams, percentages of cells with high fluorescence intensity, and percentages of cells in the lysis-resistant region were evaluated alone or in combination to establish a "leukemic flag." Sensitivity, specificity, negative (LR-) and positive (LR+) likelihood ratios, and the number of false-negative (FN) and false-positive (FP) results were calculated, and receiver operating characteristic curves were designed for numerical values. RESULTS: Among single measurements and parameters, only the evaluation of scattergrams minimized FN and FP results (sensitivity 100%, specificity 94.8%, LR+ 19.17, and LR- 0.00), although their interpretation was subjective. The more objective approach based on the generation of a "leukemic flag" had a sensitivity of 100%, specificity of 87.0%, LR- of 0.00, and LR+ of 7.67. CONCLUSION: Using a novel gating strategy the Sysmex XT-2000iV may be used effectively to screen canine blood for hematopoietic neoplasia. |
| | |
Authors:
|
Maria Elena Gelain; Gabriele Rossi; Luca Giori; Stefano Comazzi; Saverio Paltrinieri |
Related Documents
:
|
8404195 - Prediction of respiratory symptoms by peripheral blood neutrophils and eosinophils in t... 6731205 - Total blood eosinophilia in allergic (type i allergy) and non-allergic asthma, rhinitis... 12661825 - Performance evaluation of the sysmex xt-2000i automated hematology analyzer. 495565 - Pitfalls in the interpretation of leukocyte counts of newborn infants. 2558365 - Effect of neurokinin a on human temporal muscle blood flow. 17424755 - Earlier cessation of desflurane supply in closed-circuit anesthesia reduces emergence t... |
Publication Detail:
|
Type: Journal Article Date: 2010-03-10 |
Journal Detail:
|
Title: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology Volume: 39 ISSN: 0275-6382 ISO Abbreviation: Vet Clin Pathol Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-07-13 Completed Date: 2010-10-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9880575 Medline TA: Vet Clin Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 169-79 Citation Subset: IM |
Affiliation:
|
Department of Veterinary Pathology, Hygiene and Public Health, University of Milan, Milan, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Autoanalysis / veterinary Dog Diseases / blood, diagnosis* Dogs Flow Cytometry / instrumentation, veterinary* Leukemia / blood, diagnosis, veterinary* Leukocyte Count / veterinary Reproducibility of Results Sensitivity and Specificity |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of blood cells in the Leopard Cat (Prionailurus bengalensis).
Next Document: Comparison of first-opinion and second-opinion histopathology from dogs and cats with cancer: 430 ca...