Document Detail


Identification of a mutation associated with erythromycin resistance in Bordetella pertussis: implications for surveillance of antimicrobial resistance.
MedLine Citation:
PMID:  12624047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythromycin treatment failures and in vitro resistance of Bordetella pertussis have been reported on several occasions in the past few years, but the mechanism of resistance has not been described. One potential mechanism, genetic modification of the erythromycin-binding site on the 23S rRNA of the 50S ribosomal subunit, has been observed in other bacteria. To explore this possibility, we amplified the portion of the 23S rRNA gene encoding the central loop of domain V. DNA sequencing and restriction fragment length polymorphism of the PCR products showed that each of the four erythromycin-resistant B. pertussis strains tested contained an A-to-G transition mutation at position 2058 (Escherichia coli numbering) of the 23S rRNA gene. The mutation was not found in seven erythromycin-susceptible isolates tested. Two of the resistant isolates were heterozygous, containing at least one mutant copy and one wild-type copy of the 23S rRNA gene. These results indicate that erythromycin resistance in these strains is likely due to a mutation of the erythromycin-binding site in the 23S rRNA gene. Identification of the resistance mechanism will facilitate development of molecular susceptibility testing methods that can be used directly on clinical specimens in the absence of an isolate.
Authors:
J M Bartkus; B A Juni; K Ehresmann; C A Miller; G N Sanden; P K Cassiday; M Saubolle; B Lee; J Long; A R Harrison; J M Besser
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  41     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-07     Completed Date:  2003-05-02     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1167-72     Citation Subset:  IM    
Affiliation:
Minnesota Department of Health, Minneapolis. Crossroads Medical Center, Chaska, Minnesota, USA. joanne.bartkus@health.state.mn.us
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Bordetella pertussis / drug effects*,  genetics
Child
DNA, Bacterial / analysis
Drug Resistance, Bacterial / genetics*
Erythromycin / pharmacology*
Female
Heterozygote
Humans
Microbial Sensitivity Tests
Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA, Bacterial; 114-07-8/Erythromycin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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