Document Detail

Identification of a mutation associated with erythromycin resistance in Bordetella pertussis: implications for surveillance of antimicrobial resistance.
MedLine Citation:
PMID:  12624047     Owner:  NLM     Status:  MEDLINE    
Erythromycin treatment failures and in vitro resistance of Bordetella pertussis have been reported on several occasions in the past few years, but the mechanism of resistance has not been described. One potential mechanism, genetic modification of the erythromycin-binding site on the 23S rRNA of the 50S ribosomal subunit, has been observed in other bacteria. To explore this possibility, we amplified the portion of the 23S rRNA gene encoding the central loop of domain V. DNA sequencing and restriction fragment length polymorphism of the PCR products showed that each of the four erythromycin-resistant B. pertussis strains tested contained an A-to-G transition mutation at position 2058 (Escherichia coli numbering) of the 23S rRNA gene. The mutation was not found in seven erythromycin-susceptible isolates tested. Two of the resistant isolates were heterozygous, containing at least one mutant copy and one wild-type copy of the 23S rRNA gene. These results indicate that erythromycin resistance in these strains is likely due to a mutation of the erythromycin-binding site in the 23S rRNA gene. Identification of the resistance mechanism will facilitate development of molecular susceptibility testing methods that can be used directly on clinical specimens in the absence of an isolate.
J M Bartkus; B A Juni; K Ehresmann; C A Miller; G N Sanden; P K Cassiday; M Saubolle; B Lee; J Long; A R Harrison; J M Besser
Related Documents :
12019067 - Activity of the ketolide telithromycin is refractory to erm monomethylation of bacteria...
11272257 - Streptococcus pyogenes collected in torino (northwest italy) between 1983 and 1998: sur...
23737977 - Mechanistic model of rothia mucilaginosa adaptation toward persistence in the cf lung, ...
3579257 - Distribution of erythromycin esterase and rrna methylase genes in members of the family...
25493187 - Lactic acid bacteria and yeasts involved in the fermentation ofamabere amaruranu, a ken...
6785387 - Evolution in pseudomonas fluorescens.
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  41     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-07     Completed Date:  2003-05-02     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1167-72     Citation Subset:  IM    
Minnesota Department of Health, Minneapolis. Crossroads Medical Center, Chaska, Minnesota, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anti-Bacterial Agents / pharmacology*
Bordetella pertussis / drug effects*,  genetics
DNA, Bacterial / analysis
Drug Resistance, Bacterial / genetics*
Erythromycin / pharmacology*
Microbial Sensitivity Tests
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA, Bacterial; 114-07-8/Erythromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Patterns of resistance associated with integrons, the extended-spectrum beta-lactamase SHV-5 gene, a...
Next Document:  Genotypes at the internal transcribed spacers of the nuclear rRNA operon of Pneumocystis jiroveci in...