| Identification of mono-, oligo-, and polysaccharides secreted from soybean roots. | |
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MedLine Citation:
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PMID: 12884130 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The mist culture system was conducted to study secreted polysaccharides from soybean ( Glycine max) roots grown for 15 days. Roots were rinsed with distilled water (DW) for 15 min, then with 30 mM oxalic acid (OXA) for 15 min to remove ionically bound sugar. Released sugars were further fractionated into low (L) and high (H) molecular weight fractions with Sephadex G-10. DW rinsing released 190 microg neutral sugar (NS) and 62 microg uronic acid (UA) per plant, while 374 microg NS and 70 microg UA per plant were released by OXA rinsing. Acetylation analysis revealed that the L fraction by DW and OXA mainly consisted of glucose (Glc), pinitol, and UA, whereas the H fraction mainly consisted of arabinose (Ara), galactose (Gal), Glc, and UA. The presence of rhamnose (2%-6%) in both fractions suggests secretion of rhamnogalacturonans. Methylation analysis revealed that the H fraction by DW and OXA contained T-Ara, 3-, 6-, and 3,6-Gal, suggesting the presence of type II arabinogalactan and arabinogalactan proteins. HPLC analysis detected mono-, di-, and tri-GalA in the L fraction by DW and OXA. Substances corresponding to sucrose, kojibiose, cello- and laminari-oligosaccharides were also found in root exudates. |
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Authors:
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Paul Benjamin Timotiwu; Naoki Sakurai |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of plant research Volume: 115 ISSN: 0918-9440 ISO Abbreviation: J. Plant Res. Publication Date: 2002 Apr |
Date Detail:
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Created Date: 2003-07-28 Completed Date: 2003-11-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9887853 Medline TA: J Plant Res Country: Japan |
Other Details:
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Languages: eng Pagination: 77-85 Citation Subset: - |
Affiliation:
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Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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