| Identification and molecular characterization of a Brachypodium distachyon GIGANTEA gene: functional conservation in monocot and dicot plants. | |
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MedLine Citation:
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PMID: 20012169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Developmental phase change and flowering transition are emerging as potential targets for biomass agriculture in recent years. The GIGANTEA (GI) gene is one of the central regulators that direct flowering promotion and phase transition. In this work, we isolated a GI gene orthologue from the small annual grass Brachypodium distachyon inbred line Bd21 (Brachypodium), which is perceived as a potential model monocot for studies on bioenergy grass species. A partial GI gene sequence was identified from a Brachypodium expressed sequence tag library, and a full-size gene (BdGI) was amplified from a Brachypodium cDNA library using specific primer sets designed through analysis of monocot GI gene sequences. The BdGI gene was up-regulated by light and cold. A circadian rhythm set by light-dark transition also regulated the expression of the BdGI gene. The deduced amino acid sequence of the BdGI protein shares higher than 70% of sequence identity with the GI proteins in monocots and Arabidopsis. In addition, the BdGI protein is constitutively targeted to the nucleus and physically interacts with the ZEITLUPE (ZTL) and CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) proteins, like the Arabidopsis GI protein. Interestingly, heterologous expression of the BdGI gene in a GI-deficient Arabidopsis mutant rescued efficiently the late flowering phenotype. Together, our data indicate that the role of the GI gene in flowering induction is conserved in Arabidopsis and Brachypodium. It is envisioned that the GI genes of bioenergy grasses as well as Brachypodium could be manipulated to improve biomass by engineering developmental timing of phase transitions. |
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Authors:
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Shin-Young Hong; Sangmin Lee; Pil Joon Seo; Moon-Sik Yang; Chung-Mo Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-10 |
Journal Detail:
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Title: Plant molecular biology Volume: 72 ISSN: 1573-5028 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-04 Completed Date: 2010-03-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 485-97 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Seoul National University, Seoul 151-742, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics,
growth & development Arabidopsis Proteins / genetics Cell Nucleus / metabolism Circadian Rhythm / genetics Gene Expression Regulation, Plant Genes, Plant* Genetic Complementation Test Phylogeny Plant Proteins / genetics, physiology Plants, Genetically Modified Poaceae / genetics*, growth & development, physiology Species Specificity Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/GI protein, Arabidopsis; 0/Plant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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