Document Detail

Identification of the minimum essential region in the II-III loop of the dihydropyridine receptor alpha 1 subunit required for activation of skeletal muscle-type excitation-contraction coupling.
MedLine Citation:
PMID:  9578589     Owner:  NLM     Status:  MEDLINE    
We have previously shown that among several peptides encompassing various regions of the II-III loop of the dihydropyridine receptor alpha 1 subunit, only one peptide corresponding to the Thr671-Leu690 region (designated as peptide A) activated ryanodine binding to and induced calcium release from the sarcoplasmic reticulum [El-Hayek et al. (1995) J. Biol. Chem. 270, 22116-22118]. To further localize within peptide A the minimum unit essential for activating the sarcoplasmic reticulum calcium release channel, we synthesized variously truncated forms of peptide A and examined their ability to activate ryanodine binding. We found that the carboxy-terminal 10-residue region of peptide A encompassing Arg681-Leu690 (peptide As-10; s, skeletal muscle-type sequence) activated ryanodine binding in a RyR1-specific manner and induced calcium release even more efficiently than the 20-residue peptide A. Further truncation of one or more residue(s) of peptide As-10 virtually abolished both functions of activating ryanodine binding and inducing Ca2+ release. The activating ability of As-10 seems to be determined by at least two factors: (1) the distribution of the positively charged residues, and (2) the skeletal muscle-type amino acid sequence, as deduced from the comparison of various peptides with modified structures. These results provide evidence that the minimum essential unit for the in situ trigger of skeletal muscle excitation-contraction coupling is localized in the Arg681-Leu690 region of the II-III loop of the alpha 1 subunit of the dihydropyridine receptor.
R El-Hayek; N Ikemoto
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  37     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-04     Completed Date:  1998-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7015-20     Citation Subset:  IM; S    
Boston Biomedical Research Institute, Massachusetts 02114, USA.
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MeSH Terms
Amino Acid Sequence
Calcium Channels / chemistry*,  physiology*
Calcium Channels, L-Type
Hydrogen-Ion Concentration
Molecular Sequence Data
Muscle Contraction* / drug effects
Muscle Proteins / chemistry,  physiology*
Muscle, Skeletal / chemistry,  physiology*
Myocardium / chemistry
Peptide Fragments / chemical synthesis,  genetics,  pharmacology
Polylysine / pharmacology
Grant Support
Reg. No./Substance:
0/Calcium Channels; 0/Calcium Channels, L-Type; 0/Muscle Proteins; 0/Peptide Fragments; 25104-18-1/Polylysine

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