Document Detail

Identification of milk component in ancient food residue by proteomics.
MedLine Citation:
PMID:  22615887     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Proteomic approaches based on mass spectrometry have been recently used in archaeological and art researches, generating promising results for protein identification. Little information is known about eastward spread and eastern limits of prehistoric milking in eastern Eurasia.
METHODOLOGY/PRINCIPAL FINDING: In this paper, an ancient visible food remain from Subeixi Cemeteries (cal. 500 to 300 years BC) of the Turpan Basin in Xinjiang, China, preliminarily determined containing 0.432 mg/kg cattle casein with ELISA, was analyzed by using an improved method based on liquid chromatography (LC) coupled with MALDI-TOF/TOF-MS to further identify protein origin. The specific sequence of bovine casein and the homology sequence of goat/sheep casein were identified.
CONCLUSIONS/SIGNIFICANCE: The existence of milk component in ancient food implies goat/sheep and cattle milking in ancient Subeixi region, the furthest eastern location of prehistoric milking in the Old World up to date. It is envisioned that this work provides a new approach for ancient residue analysis and other archaeometry field.
Chuan Hong; Hongen Jiang; Enguo Lü; Yunfei Wu; Lihai Guo; Yongming Xie; Changsui Wang; Yimin Yang
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Publication Detail:
Type:  Historical Article; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-16
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-22     Completed Date:  2013-01-08     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e37053     Citation Subset:  IM    
Laboratory of Human Evolution, Institute of Vertebrate Paleontology and Paleoanthropology, CAS, Beijing, China.
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MeSH Terms
Archaeology / methods
Caseins / chemistry
Chromatography, Liquid / methods
Food Analysis / methods
History, Ancient
Milk / chemistry*
Milk Proteins / analysis*,  chemistry*
Proteomics / methods
Spectrometry, Mass, Electrospray Ionization / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Reg. No./Substance:
0/Caseins; 0/Milk Proteins

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