| Identification of microRNA expression patterns and definition of a microRNA/mRNA regulatory network in distinct molecular groups of multiple myeloma. | |
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MedLine Citation:
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PMID: 19846888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To date, little evidence of miRNA expression/deregulation in multiple myeloma has been reported. To characterize miRNA in the context of the major multiple myeloma molecular types, we generated miRNA expression profiles of highly purified malignant plasma cells from 40 primary tumors. Furthermore, transcriptional profiles, available for all patients, were used to investigate the occurrence of miRNA/predicted target mRNA pair anticorrelations, and the miRNA and genome-wide DNA data were integrated in a subset of patients to evaluate the influence of allelic imbalances on miRNA expression. Differential miRNA expression patterns were identified, which were mainly associated with the major IGH translocations; particularly, t(4;14) patients showed specific overexpression of let-7e, miR-125a-5p, and miR-99b belonging to a cluster at 19q13.33. The occurrence of other lesions (ie, 1q gain, 13q and 17p deletions, and hyperdiploidy) was slightly characterized by specific miRNA signatures. Furthermore, the occurrence of several allelic imbalances or loss of heterozygosity was found significantly associated with the altered expression of miRNAs located in the involved regions, such as let-7b at 22q13.31 or miR-140-3p at 16q22. Finally, the integrative analysis based on computational target prediction and miRNA/mRNA profiling defined a network of putative functional miRNA-target regulatory relations supported by expression data. |
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Authors:
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Marta Lionetti; Marta Biasiolo; Luca Agnelli; Katia Todoerti; Laura Mosca; Sonia Fabris; Gabriele Sales; Giorgio Lambertenghi Deliliers; Silvio Bicciato; Luigia Lombardi; Stefania Bortoluzzi; Antonino Neri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Blood Volume: 114 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-01-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: e20-6 Citation Subset: AIM; IM |
Affiliation:
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Department of Medical Sciences, University of Milan, Milan, Italy. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE13591; GSE16121; GSE17498 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Gene Dosage Gene Expression Profiling* Gene Regulatory Networks* Genetic Variation Genome, Human Genome-Wide Association Study Humans Loss of Heterozygosity MicroRNAs / genetics* Multiple Myeloma / genetics*, pathology Oligonucleotide Array Sequence Analysis / methods Plasma Cells / metabolism, pathology RNA, Messenger / genetics* Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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