Document Detail


Identification of metabolites in WZS-miniature pig urine after oral administration of Danshen decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry.
MedLine Citation:
PMID:  23212729     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Danshen (DS) is a widely used traditional Chinese medicine for treating cardiovascular and cerebrovascular diseases. A simple, rapid and sensitive method was developed for identification of the in vivo metabolites in urine of WZS-miniature pigs after oral administration of DS decoction by HPLC coupled with diode array detection with electrospray ionization tandem ion trap and time-of-flight mass spectrometry. This method has been successfully applied to simultaneous identification of 50 compounds (including 11 new ones) in pig urine. In addition, one new compound, (3-hydroxyphenyl) crylic acid glycine methyl ester (C1), along with eight known ones were first isolated by column chromatography and identified by spectroscopic means, including 1D/2DNMR and mass spectrometry, as reference substances. Ten phenolic compounds (protocatechuic aldehyde, protocatechuic acid, caffeic acid, danshensu, ferulic acid, isoferulic acid, rosmarinic acid and salvianolic acid A/B/D) were found to be the main absorbed original constituents of DS decoction, which underwent the metabolic reactions of glucuronidation, sulfation, methylation, hydrogenation and glycine conjugation in vivo. In conclusion, the developed method is applicable to the analysis and identification of constituents in biological matrices after administration of DS decoction. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Xin Zhao; Dong-Hui Yang; Qi-Le Zhou; Feng Xu; Lei Zhang; Jing Liang; Guang-Xue Liu; Shao-Qing Cai; Xiu-Wei Yang
Related Documents :
24890649 - Simultaneous determination of seven phthalic acid esters in beverages using ultrasound ...
24573809 - Isotope-dilution mass spectrometric quantification of the prodrug lisdexamfetamine in h...
23665079 - Measurement of dehydroepiandrosterone sulphate (dheas): a comparison of isotope-dilutio...
10701909 - Simultaneous assay of ephedrine hydrochloride, theophylline, papaverine hydrochloride a...
23138519 - Simultaneous determination of methotrexate, dasatinib and its active metabolite n- desh...
25038769 - Simultaneous analysis of amphetamine-type stimulants in plasma by solid-phase microextr...
21703629 - Preparation and characterization of lauryl methacrylate-based monolithic microbore colu...
25175339 - Preservatives in cosmetics in the israeli market conform well to the eu legislation.
10532639 - A comparison of 11c-labeled l-dopa and l-fluorodopa as positron emission tomography tra...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  -     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, The People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Impacts of Land Use Change on Malaria Vector Abundance in a Water-Limited, Highland Region of Et...
Next Document:  Periloss dissociation, symptom severity, and treatment response in complicated grief.