Document Detail


Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1.
MedLine Citation:
PMID:  8481006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mycobacterium sp. strain PYR-1, previously shown to extensively mineralize high-molecular-weight polycyclic aromatic hydrocarbons in pure culture and in sediments, degrades fluoranthene to 9-fluorenone-1-carboxylic acid. In this study, 10 other fluoranthene metabolites were isolated from ethyl acetate extracts of the culture medium by thin-layer and high-performance liquid chromatographic methods. On the basis of comparisons with authentic compounds by UV spectrophotometry and thin-layer chromatography as well as gas chromatography-mass spectral and proton nuclear magnetic resonance spectral analyses, the metabolites were identified as 8-hydroxy-7-methoxyfluoranthene, 9-hydroxyfluorene, 9-fluorenone, 1-acenaphthenone, 9-hydroxy-1-fluorenecarboxylic acid, phthalic acid, 2-carboxybenzaldehyde, benzoic acid, phenylacetic acid, and adipic acid. Authentic 9-hydroxyfluorene and 9-fluorenone were metabolized by Mycobacterium sp. strain PYR-1. A pathway for the catabolism of fluoranthene by Mycobacterium sp. strain PYR-1 is proposed.
Authors:
I Kelley; J P Freeman; F E Evans; C E Cerniglia
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  59     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-05-27     Completed Date:  1993-05-27     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  800-6     Citation Subset:  IM    
Affiliation:
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Chromatography, High Pressure Liquid
Fluorenes / metabolism*
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Mycobacterium / metabolism*
Soil Pollutants / metabolism*
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Fluorenes; 0/Soil Pollutants; 206-44-0/fluoranthene
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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