Document Detail

Identification of matrix metalloproteinases in canine cutaneous mast cell tumors.
MedLine Citation:
PMID:  11110378     Owner:  NLM     Status:  MEDLINE    
Presence of matrix metalloproteinases has been associated with tumor invasion and metastasis in human neoplasia. The presence of matrix metalloproteinase 2 and matrix metalloproteinase 9 was determined in canine mast cell tumor tissue and normal stromal tissue from 24 dogs with spontaneously occurring cutaneous mast cell tumors. Seventeen of the mast cell tumors were of histologic grade 2, and 7 were of histologic grade 3. Gelatin zymography and computer assisted densitometry image analysis were used to quantify matrix metalloproteinase concentration. Bands from canine tissues migrated in the same location as human proenzyme and active enzyme matrix metalloproteinase 2 and matrix metalloproteinase 9 standards. A semiquantitative value for each patient sample was obtained by comparing the optical assessment density of each unknown band to the optical density of the human standard. The presence of matrix metalloproteinase 2 and matrix metalloproteinase 9 in histologic grade 2 mast cell tumors and histologic grade 3 mast cell tumors was compared, as was presence of matrix metalloproteinases in tumor and stromal tissue. There was dramatically more proenzyme matrix metalloproteinase 9 activity in histologic grade 3 mast cell tumors when compared to grade 2 tumors (P = .03). There was also dramatically more active enzyme matrix metalloproteinase 2 and active enzyme matrix metalloproteinase 9 activity in tumor tissue compared to stromal tissue (P = .02, P < .0001). This study demonstrates that the proenzyme and active enzyme forms of matrix metalloproteinase 2 and matrix metalloproteinase 9 are present in canine mast cell tumors. This appears to be related to the degree of histologic malignancy, although histologic grade 1 tumors were not evaluated.
N F Leibman; S E Lana; R A Hansen; B E Powers; M J Fettman; S J Withrow; G K Ogilvie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of veterinary internal medicine / American College of Veterinary Internal Medicine     Volume:  14     ISSN:  0891-6640     ISO Abbreviation:  J. Vet. Intern. Med.     Publication Date:    2000 Nov-Dec
Date Detail:
Created Date:  2001-02-28     Completed Date:  2001-04-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8708660     Medline TA:  J Vet Intern Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  583-6     Citation Subset:  IM    
Animal Cancer Center, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, USA.
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MeSH Terms
Dog Diseases / enzymology*,  pathology
Electrophoresis, Agar Gel / veterinary
Image Processing, Computer-Assisted
Mast-Cell Sarcoma / enzymology,  pathology,  veterinary*
Matrix Metalloproteinase 2 / analysis*,  chemistry
Matrix Metalloproteinase 9 / analysis*,  chemistry
Skin Neoplasms / enzymology,  pathology,  veterinary*
Statistics, Nonparametric
Grant Support
2 PO-1 CA 29582/CA/NCI NIH HHS
Reg. No./Substance:
EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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