| Identification and mapping of a novel blackleg resistance locus LepR4 in the progenies from Brassica napus × B. rapa subsp. sylvestris. | |
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MedLine Citation:
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PMID: 22733446 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Blackleg, caused by Leptosphaeria maculans, is one of the most economically important diseases of Brassica napus worldwide. Two blackleg-resistant lines, 16S and 61446, were developed through interspecific hybridization between B. napus and B. rapa subsp. sylvestris and backcrossing to B. napus. Classical genetic analysis demonstrated that a single recessive gene in both lines conferred resistance to L. maculans and that the resistance alleles were allelic. Using BC(1) progeny derived from each resistant plant, this locus was mapped to B. napus linkage group N6 and was flanked by microsatellite markers sN2189b and sORH72a in an interval of about 10 cM, in a region equivalent to about 6 Mb of B. rapa DNA sequence. This new resistance gene locus was designated as LepR4. The two lines were evaluated for resistance to a wide range of L. maculans isolates using cotyledon inoculation tests under controlled environment conditions, and for stem canker resistance in blackleg field nurseries. Results indicated that line 16S, carrying LepR4a, was highly resistant to all isolates tested on cotyledons and had a high level of stem canker resistance under field conditions. Line 61446, carrying LepR4b, was only resistant to some of the isolates tested on cotyledons and was weakly resistant to stem canker under field conditions. |
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Authors:
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Fengqun Yu; Richard K Gugel; H Randy Kutcher; Gary Peng; S Roger Rimmer |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-26 |
Journal Detail:
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Title: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik Volume: - ISSN: 1432-2242 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0145600 Medline TA: Theor Appl Genet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada, fengqun.yu@agr.gc.ca. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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