Document Detail


Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
MedLine Citation:
PMID:  12584197     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transactivators. We recently identified a mammalian homologue of yeast Mediator subunit Med8 and partially purified a Med8-containing Mediator complex from rat liver nuclei (Brower, C. S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R. D., Malik, S., Lane, W. S., Sorokina, I., Roeder, R. G., Conaway, J. W., and Conaway, R. C. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10353-10358). Analysis of proteins present in the most highly purified Med8-containing fractions by tandem mass spectrometry led to the identification of many known mammalian Mediator subunits, as well as four potential Mediator subunits exhibiting sequence similarity to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. Here we present direct biochemical evidence that these four proteins are bona fide mammalian Mediator subunits. In addition, we identify direct pairwise binding partners of these proteins among the known mammalian Mediator subunits. Taken together, our findings identify a collection of novel mammalian Mediator subunits and shed new light on the underlying architecture of the mammalian Mediator complex.
Authors:
Shigeo Sato; Chieri Tomomori-Sato; Charles A S Banks; Irina Sorokina; Tari J Parmely; Stephanie E Kong; Jingji Jin; Yong Cai; William S Lane; Christopher S Brower; Ronald C Conaway; Joan Weliky Conaway
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-02-12
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  278     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-21     Completed Date:  2003-07-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15123-7     Citation Subset:  IM    
Affiliation:
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Liver / chemistry
Mass Spectrometry
Mediator Complex
Precipitin Tests
Protein Binding
Protein Subunits / chemistry,  isolation & purification,  metabolism
RNA Polymerase II
Rats
Saccharomyces cerevisiae Proteins
Sequence Homology
Trans-Activators / chemistry*,  isolation & purification,  metabolism
Transcription Factors / chemistry,  isolation & purification,  metabolism
Grant Support
ID/Acronym/Agency:
R37 GM41628/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/MED8 protein, S cerevisiae; 0/Mediator Complex; 0/Protein Subunits; 0/ROX3 protein, S cerevisiae; 0/SRB5 protein, S cerevisiae; 0/SRB6 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; 0/Transcription Factors; EC 2.7.7.-/RNA Polymerase II

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