Document Detail


Identification of malic acid from yeast.
MedLine Citation:
PMID:  5640383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Evidence indicated an accumulation of l-malic acid in the synthetic growth medium of both lysine auxotrophs and wild-type yeast. Malic acid was isolated from growth experiments and was identified by paper and column chromatography, by comparison of infrared spectra with authentic l-malic acid, as well as enzymatically, as a substrate of malic dehydrogenase. Malic acid was accumulated when glucose was used as a carbon source. This did not occur when sodium lactate was the carbon source. Malic, succinic, and citric acids, and a few other as yet unidentified organic acids, were accumulated in the culture supernatant fluid when yeast was grown in a chemically defined medium. The accumulation of these acids had no obvious relation to the pathway for lysine biosynthesis.
Authors:
J K Bhattacharjee; M E Maragoudakis; M Strassman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  95     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1968 Feb 
Date Detail:
Created Date:  1968-05-03     Completed Date:  1968-05-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  495-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Carbon Isotopes
Chromatography
Chromatography, Paper
Citrates / analysis
Glucose / metabolism
Lactates / metabolism
Lysine / metabolism
Malates / analysis*
Mutation
Saccharomyces / analysis*,  metabolism
Succinates / analysis
Chemical
Reg. No./Substance:
0/Acetates; 0/Carbon Isotopes; 0/Citrates; 0/Lactates; 0/Malates; 0/Succinates; 50-99-7/Glucose; 56-87-1/Lysine
Comments/Corrections

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