| Identification of the main promoter directing cereulide biosynthesis in emetic Bacillus cereus and its application for real-time monitoring of ces gene expression in foods. | |
| | |
MedLine Citation:
|
PMID: 20038713 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cereulide, the emetic Bacillus cereus toxin, is synthesized by cereulide synthetase via a nonribosomal peptide synthetase (NRPS) mechanism. Previous studies focused on the identification, structural organization, and biochemical characterization of the ces gene locus encoding cereulide synthetase; however, detailed information about the transcriptional organization of the ces genes was lacking. The present study shows that the cesPTABCD genes are transcribed as a 23-kb polycistronic transcript, while cesH, encoding a putative hydrolase, is transcribed from its own promoter. Transcription initiation was mapped by primer extension and rapid amplification of cDNA ends (RACE). Deletion analysis of promoter elements revealed a main promoter located upstream of the cesP coding sequence, encoding a 4'-phosphopantetheinyl transferase. This promoter drives transcription of cesPTABCD. In addition, intracistronic promoter regions in proximity to the translational start sites of cesB and cesT were identified but were only weakly active under the chosen assay conditions. The identified main promoter was amplified from the emetic reference strain B. cereus F4810/72 and fused to luciferase genes in order to study promoter activity in complex environments and to establish a biomonitoring system to assess cereulide production in different types of foods. ces promoter activity was strongly influenced by the food matrix and varied by 5 orders of magnitude. The amount of cereulide toxin extracted from spiked foods correlated well with the bioluminescence data, thus illustrating the potential of the established reporter system for monitoring of ces gene expression in complex matrices. |
| | |
Authors:
|
Monica K Dommel; Elrike Frenzel; Bernd Strasser; Claudia Blöchinger; Siegfried Scherer; Monika Ehling-Schulz |
Related Documents
:
|
11678883 - Keratinocyte gene therapy: inducible promoters and in vivo control of transgene express... 15875723 - Isolation and characterization of a green tissue-specific promoter from pigeonpea [caja... 10448063 - Chop/gadd153 and methionyl-trna synthetase (metrs) genes overlap in a conserved region ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-28 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-02-08 Completed Date: 2010-04-02 Revised Date: 2010-09-27 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 1232-40 Citation Subset: IM |
Affiliation:
|
Microbiology Unit, Center for Nutrition and Food Research ZIEL, Technische Universität München, 85350 Freising, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Bacillus cereus / genetics*, growth & development, metabolism, pathogenicity* Bacterial Toxins / biosynthesis*, toxicity Base Sequence DNA Primers / genetics DNA, Bacterial / genetics Depsipeptides / biosynthesis*, toxicity Environmental Monitoring / methods Food Microbiology* Foodborne Diseases / etiology Gene Expression Genes, Bacterial Genes, Reporter Humans Molecular Sequence Data Multigene Family Peptide Synthases / genetics, metabolism Promoter Regions, Genetic Vomiting / etiology |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Toxins; 0/DNA Primers; 0/DNA, Bacterial; 0/Depsipeptides; 0/cereulide; EC 6.3.2.-/Peptide Synthases; EC 6.3.2.-/non-ribosomal peptide synthase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of Growth Phase Feeding Strategies on Succinate Production by Metabolically Engineered E. col...
Next Document: Compounds from an unbiased chemical screen reverse both ER-to-Golgi trafficking defects and mitochon...