Document Detail

Identification of macrosialin (CD68) on the surface of host macrophages as the receptor for the intercellular adhesive molecule (ICAM-L) of Leishmania amazonensis.
MedLine Citation:
PMID:  19540239     Owner:  NLM     Status:  MEDLINE    
The intercellular adhesive molecule, ICAM-L, of Leishmania amazonensis is known to block the attachment as well as internalisation of Leishmania for infection in host macrophages. We employed monoclonal antibodies (mAb) to the surface molecules of a macrophage to block the attachment of ICAM-L to the macrophage surface and identified that CD68 macrosialin is likely the receptor molecule on the macrophage for ICAM-L. We then demonstrated physical interaction between ICAM-L and macrosialin by co-immunoprecipitation of macrosialin with ICAM-L or vice versa. Finally, macrosialin is expressed in macrosialin-negative murine fibroblast cell line NCTC clone 2555 and demonstrates that both ICAM-L and promastigotes of L. amazonensis can bind to the CD68 transfectant. We thus conclude that CD68 macrosialin is the receptor on host macrophages for ICAM-L. Also, involvement of ICAM-L-macrosialin interaction in other Leishmania species and other mammalian macrophages were demonstrated, indicating the biological relevance of this ligand-receptor interaction.
Hang-Pong Ng; Sui-Chi Chiang; Yi Chi; Sho-Tone Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-18
Journal Detail:
Title:  International journal for parasitology     Volume:  39     ISSN:  1879-0135     ISO Abbreviation:  Int. J. Parasitol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-12     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0314024     Medline TA:  Int J Parasitol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1539-50     Citation Subset:  IM    
Division of Infectious Disease and Immunology, Academia Sinica, (IMBS) No. 128, Academia Road Sec. 2, Nan-Kang, Taipei 11529, Taiwan, ROC.
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MeSH Terms
Antigens, CD / chemistry,  immunology*,  metabolism
Antigens, Differentiation, Myelomonocytic / chemistry,  immunology*,  metabolism
Cell Adhesion / physiology*
Cell Adhesion Molecules / chemistry,  immunology*,  metabolism
Leishmania / metabolism*
Macrophages / chemistry*,  parasitology
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 protein, mouse; 0/Cell Adhesion Molecules

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