| Identification of the luciferin-luciferase system and quantification of coelenterazine by mass spectrometry in the deep-sea luminous ostracod Conchoecia pseudodiscophora. | |
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MedLine Citation:
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PMID: 15515099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bioluminescence system of the ostracod Conchoecia pseudodiscophora, which is abundant in the Sea of Japan, has been characterized. The luminescence (lambda(max)=463 nm) is produced by a luciferin-luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramide, and coelenteramine from C. pseudodiscophora were quantified by LC-ESI-MS/MS analysis. The coelenterazine content was estimated to be approximately 230 pg per animal by using a calibration curve of synthetic coelenterazine. The reaction between homogenates of C. pseudodiscophora and synthetic coelenterazine showed luminescence activity; this suggests that a coelenterazine-type luciferase is present. |
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Authors:
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Yuichi Oba; Hiroshi Tsuduki; Shin-ichi Kato; Makoto Ojika; Satoshi Inouye |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chembiochem : a European journal of chemical biology Volume: 5 ISSN: 1439-4227 ISO Abbreviation: Chembiochem Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-11-10 Completed Date: 2005-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: Germany |
Other Details:
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Languages: eng Pagination: 1495-9 Citation Subset: IM |
Affiliation:
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Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Crustacea / chemistry* Firefly Luciferin / chemistry, metabolism* Imidazoles / analysis*, chemistry, metabolism* Luciferases / chemistry, metabolism* Luminescent Measurements* Mass Spectrometry / methods Molecular Structure Pyrazines / analysis*, chemistry, metabolism* Species Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Imidazoles; 0/Pyrazines; 2591-17-5/Firefly Luciferin; 55779-48-1/coelenterazine; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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