| Identification of long-chain fatty acids and alcohols from human cerumen by the use of picolinyl and nicotinate esters. | |
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MedLine Citation:
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PMID: 2790258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human cerumen was hydrolysed with base and the constituents were examined as trimethylsilyl (TMS), methyl ester/TMS, picolinyl/TMS and nicotinate/TMS derivatives by gas chromatography and gas chromatography/mass spectrometry. A sample was also reacted with osmium tetroxide for double bond location. The major constituents were cholesterol, squalene and several series of long-chain fatty acids and alcohols. These latter compounds had chain lengths of 12-26 carbon atoms and were predominantly either straight-chain saturated or straight-chain unsaturated compounds. Saturated branched-chain acids with methyl groups predominantly on even-numbered carbon atoms were present but were less abundant. Unsaturated, branched-chain acids were also present. The major unsaturated acids contained unsaturation at the delta-6-position or were derived from these acids by chain elongation. The compounds were similar to those found in vernix caseosa. The mass spectra of picolinyl esters were, for the first time, shown to be capable of determining both the position of unsaturation and methyl branching in the same molecule. |
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Authors:
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D J Harvey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical & environmental mass spectrometry Volume: 18 ISSN: 0887-6134 ISO Abbreviation: Biomed. Environ. Mass Spectrom. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-11-17 Completed Date: 1989-11-17 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 8603224 Medline TA: Biomed Environ Mass Spectrom Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 719-23 Citation Subset: IM |
Affiliation:
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University Department of Pharmacology, Oxford, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cerumen / analysis* Chromatography, Gas Fatty Acids / analysis* Fatty Acids, Unsaturated / analysis Fatty Alcohols / analysis* Gas Chromatography-Mass Spectrometry Humans Male Middle Aged Nicotinic Acids / analysis Picolinic Acids / analysis Reference Standards |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Fatty Alcohols; 0/Nicotinic Acids; 0/Picolinic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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