Document Detail

Identification of leukemia cell-derived inhibitory activity (LIA) in conditioned media from human myeloid leukemic cell line ML-2.
MedLine Citation:
PMID:  3484646     Owner:  NLM     Status:  MEDLINE    
Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5 X 10(6) ML-2 cells/ml or 1 X 10(6) ML-2 cells/ml in serum-free RPMI for 5 or 24 hours respectively, and was isolated by ultrafiltration through an XM 300 Diaflo membrane followed by chromatography on Sepharose 6 B. Ferritin, prepared from human placenta, had the same inhibitory effect on CFU-GM. Antibodies against human placental ferritin completely inactivated the inhibitory effect of both human placental ferritin and the factor released from ML-2 cells. The inhibitory activity produced by the cell-line ML-2 was considered as LIA (leukemia cell-derived inhibitory activity) earlier found in HL-60 cell line and AML and CML cells.
V Cukrová; Z Hrkal; H Koprivová; J Neuwirt
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Blut     Volume:  52     ISSN:  0006-5242     ISO Abbreviation:  Blut     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-03-10     Completed Date:  1986-03-10     Revised Date:  2008-08-15    
Medline Journal Info:
Nlm Unique ID:  0173401     Medline TA:  Blut     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  51-8     Citation Subset:  IM    
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MeSH Terms
Bone Marrow
Cell Line
Chromatography, Gel
Colony-Forming Units Assay
Colony-Stimulating Factors / analysis,  antagonists & inhibitors*
Culture Media / analysis*
Ferritins / pharmacology
Hematopoietic Stem Cells
Isoelectric Focusing
Leukemia, Myeloid*
Reg. No./Substance:
0/Colony-Stimulating Factors; 0/Culture Media; 0/acidic isoferritin; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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