| Identification of leukemia cell-derived inhibitory activity (LIA) in conditioned media from human myeloid leukemic cell line ML-2. | |
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MedLine Citation:
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PMID: 3484646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5 X 10(6) ML-2 cells/ml or 1 X 10(6) ML-2 cells/ml in serum-free RPMI for 5 or 24 hours respectively, and was isolated by ultrafiltration through an XM 300 Diaflo membrane followed by chromatography on Sepharose 6 B. Ferritin, prepared from human placenta, had the same inhibitory effect on CFU-GM. Antibodies against human placental ferritin completely inactivated the inhibitory effect of both human placental ferritin and the factor released from ML-2 cells. The inhibitory activity produced by the cell-line ML-2 was considered as LIA (leukemia cell-derived inhibitory activity) earlier found in HL-60 cell line and AML and CML cells. |
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Authors:
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V Cukrová; Z Hrkal; H Koprivová; J Neuwirt |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Blut Volume: 52 ISSN: 0006-5242 ISO Abbreviation: Blut Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-03-10 Completed Date: 1986-03-10 Revised Date: 2008-08-15 |
Medline Journal Info:
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Nlm Unique ID: 0173401 Medline TA: Blut Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 51-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Cell Line Chromatography, Gel Colony-Forming Units Assay Colony-Stimulating Factors / analysis, antagonists & inhibitors* Culture Media / analysis* Ferritins / pharmacology Granulocytes Hematopoiesis Hematopoietic Stem Cells Humans Isoelectric Focusing Leukemia, Myeloid* Macrophages Male Mice Placenta |
| Chemical | |
Reg. No./Substance:
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0/Colony-Stimulating Factors; 0/Culture Media; 0/acidic isoferritin; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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