| Identification of the last unknown genes in the fermentation pathway of lysine. | |
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MedLine Citation:
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PMID: 17166837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the proteins of the lysine fermentation pathway were biochemically characterized more than thirty years ago, the genes encoding the proteins that catalyze three steps of this pathway are still unknown. We combined gene context, similarity of enzymatic mechanisms, and molecular weight comparisons with known proteins to select candidate genes for these three orphan proteins. We used a wastewater metagenomic collection of sequences to find and characterize the missing genes of the lysine fermentation pathway. After recombinant protein production and purification following cloning in Escherichia coli, we demonstrated that these genes (named kdd, kce, and kal) encode a l-erythro-3,5-diaminohexanoate dehydrogenase, a 3-keto-5-aminohexanoate cleavage enzyme, and a 3-aminobutyryl-CoA ammonia lyase, respectively. Because all of the genes of the pathway are now identified, we used this breakthrough to detect lysine-fermenting bacteria in sequenced genomes. We identified twelve bacteria that possess these genes and thus are expected to ferment lysine, and their gene organization is discussed. |
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Authors:
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Annett Kreimeyer; Alain Perret; Christophe Lechaplais; David Vallenet; Claudine Médigue; Marcel Salanoubat; Jean Weissenbach |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-13 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-05 Completed Date: 2007-04-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7191-7 Citation Subset: IM |
Affiliation:
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CNRS-UMR 8030, Genoscope, 91057 Evry Cedex, France. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/CU151840; CU151841; CU151896 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Oxidoreductases
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genetics* Ammonia-Lyases / genetics* Bacteria / metabolism* Base Sequence Fermentation* Genes, Bacterial* Lysine / metabolism* Molecular Sequence Data Multigene Family NADP / metabolism Oxo-Acid-Lyases / genetics* |
| Chemical | |
Reg. No./Substance:
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53-59-8/NADP; 56-87-1/Lysine; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.1.11/L-erythro-3,5-diaminohexanoate dehydrogenase; EC 4.1.3.-/3-keto-5-aminohexanoate cleavage enzyme; EC 4.1.3.-/Oxo-Acid-Lyases; EC 4.3.1.-/3-aminobutyryl-CoA deaminase; EC 4.3.1.-/Ammonia-Lyases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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