| Identification of a laminin-like substance on the surface of high-malignant murine fibrosarcoma cells. | |
| | |
MedLine Citation:
|
PMID: 6325478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
High- and low-malignant murine fibrosarcoma cells were stained with anti-laminin antibodies using immunoperoxidase techniques and examined by electron microscopy. With the high-malignant cells, specific staining was observed along the cell surface. Use of normal rabbit serum in place of the rabbit anti-laminin or pretreatment of the anti-laminin with soluble laminin completely eliminated this staining. No immunoperoxidase staining was observed with the low-malignant cells. In additional studies, membrane fractions were prepared from the high- and low-malignant cells and used to immunize rabbits. The animals immunized with the membrane fractions from the high-malignant cells produced antibodies that reacted by enzyme-linked immunosorbent assay (ELISA) with murine laminin obtained from the EHS sarcoma. The animals immunized with membrane fractions from the low-malignant cells did not. These studies provide strong evidence that the high-malignant cells (but not the low) express on their cell surface a substance that is immunologically cross-reactive with laminin. In addition, the high-malignant cells (but not the low) secreted a material into the cell culture fluid that could be specifically immunoprecipitated with antilaminin antibodies. The immunoprecipitated material co-migrated with purified laminin when examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis under reducing conditions. The existence of this substance associated with the surface of the high-malignant cells and its absence from that of the low-malignant cells may explain the previously noted difference between these cells in their ability to attach to type IV collagen. This difference may also contribute to the dissimilarity between these cells in their metastatic potential. |
| | |
Authors:
|
J P McCoy; R V Lloyd; M S Wicha; J Varani |
Related Documents
:
|
22511458 - Quantitative proteomics profiling of murine mammary gland cells unravels impact of anne... 22661928 - Modulation of iron metabolism in aging and in alzheimer's disease: relevance of the cho... 22912728 - Cell surface cdc37 participates in extracellular hsp90 mediated cancer cell invasion. 21094138 - Centchroman inhibits proliferation of head and neck cancer cells through the modulation... 18278448 - Construction of recombinant adenovirus carrying grim19 and its effect on sw480 cells. 20865008 - A comprehensive molecular interaction map of the budding yeast cell cycle. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of cell science Volume: 65 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 1984 Jan |
Date Detail:
|
Created Date: 1984-06-01 Completed Date: 1984-06-01 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 139-51 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Neoplasm / immunology Antigen-Antibody Reactions Enzyme-Linked Immunosorbent Assay Fibrosarcoma / analysis*, ultrastructure Immunoenzyme Techniques Laminin / analysis*, immunology Membrane Proteins / analysis Mice Mice, Inbred C57BL Microscopy, Electron Sarcoma, Experimental / analysis alpha-Galactosidase / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
CA29550/CA/NCI NIH HHS; CA29551/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Neoplasm; 0/Laminin; 0/Membrane Proteins; EC 3.2.1.22/alpha-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differential effect of arginine modification with 1,2-cyclohexanedione on the capacity of vimentin a...
Next Document: Control of cell mobility by cyclic AMP.