| Identification of an l-alanine export system in Escherichia coli and isolation and characterization of export-deficient mutants. | |
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MedLine Citation:
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PMID: 21208269 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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An Escherichia coli strain that exhibits a double auxotrophy for l-alanine and d-alanine was constructed. During growth in the presence of the dipeptide l-alanyl-l-alanine (Ala-Ala), this was fully consumed with concomitant extracellular accumulation of l-alanine in a twofold molar concentration compared with the dipeptide. This finding indicates that the strain not only can hardly degrade l-alanine but has an export system(s) for l-alanine. To obtain access to the system, we chemically mutagenized the l-alanine-nonmetabolizing strain and isolated mutants with increased Ala-Ala sensitivity. Two such mutants accumulated l-alanine up to 150-190 mM in the cytoplasm with a reduced rate of l-alanine export relative to the parent strain in the presence of Ala-Ala. Furthermore, when chloramphenicol was added together with Ala-Ala, the parent strain accumulated l-alanine in the cytoplasm to a level similar to that observed in the mutants in the absence of chloramphenicol. In contrast, the intracellular l-alanine level in the mutants did not change irrespective of chloramphenicol treatment. From these results, we conclude that E. coli has an inducible l-alanine export carrier, together with a second, as yet unidentified, mechanism of alanine export. |
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Authors:
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Hatsuhiro Hori; Tasuke Ando; Emiko Isogai; Hiroshi Yoneyama; Ryoichi Katsumata |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-5 |
Journal Detail:
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Title: FEMS microbiology letters Volume: - ISSN: 1574-6968 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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