| Identification of kinase substrates by bimolecular complementation assays. | |
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MedLine Citation:
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PMID: 22098373 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Due to the transient nature of kinase-substrate interactions, the detection of kinase targets, although central for understanding many biological processes, has remained challenging. Here we present a straightforward procedure that relies on the comparison of wild type with activation-loop mutants in the kinase of interest by bimolecular complementation assays. As a proof of functionality, we present the identification and in vivo confirmation of substrates of the major cell-cycle kinase in Arabidopsis revealing a direct link between cell proliferation and the control of the redox state. |
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Authors:
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Stefan Pusch; Hirofumi Harashima; Arp Schnittger |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-21 |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: - ISSN: 1365-313X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd. |
Affiliation:
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Unigruppe am Max-Planck-Institut für Züchtungsforschung, Max-Delbrück-Laboratorium, Lehrstuhl für Botanik III, Universität Köln, Carl-von-Linné-Weg 10, D-50829 Köln, Germany Present address: Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany Institut de Biologie Moléculaire des Plantes (IBMP), UPR2357 du CNRS, 12, rue du Général Zimmer, 67084 Strasbourg, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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