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Identification of key proteins of cultured human intestinal cells involved in interaction with enteroaggregative Escherichia coli.
MedLine Citation:
PMID:  22672426     Owner:  NLM     Status:  Publisher    
Enteroaggregative Escherichia coli (EAEC) adherence to human intestinal tissue is known to be mediated by aggregative adherence fimbriae (AAF). However, the host cell molecules involved in EAEC adherence remain uncharacterized. In the present study, four key membrane glycoproteins of cultured human intestinal epithelial cells (INT-407) were identified, which were found to be involved in interaction with T8 strain of EAEC. Nine membrane proteins of INT-407 cells were found to interact with EAEC-T8 of which four were identified as fibronectin, epidermal growth factor receptor (EGFR), Thrombospondin-1 (TSP1) and glucose regulated protein (GRP-94). Our findings were validated in Western blot using antibody against each identified protein. The adherence of EAEC-T8 to INT-407 cells was reduced to ~26%, ~29%, ~37%, ~76% in presence of the antibody against GRP-94, EGFR, fibronectin and TSP-1, respectively. These findings were further substantiated by flow cytometry where the final mean fluorescence intensity value of the INT-407 cells (~1075) resulting from adherent labelled bacteria was found to be reduced to ~ 26, ~ 132, ~ 228 and ~ 597 in presence of antibody against GRP-94, EGFR, fibronectin and TSP-1. We propose that GRP- 94, EGFR, fibronectin and TSP-1 are involved in the aggregative adherence of EAEC-T8 to INT-407 cells. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Monica Konar; Oshima Sachin; Anshu Priya; Sujata Ghosh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-2
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  -     ISSN:  1574-695X     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Department of Experimental Medicine & Biotechnology, Post Graduate Institute of Medical Education & Research, Chandigarh, India.
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