Document Detail

Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain.
MedLine Citation:
PMID:  12627226     Owner:  NLM     Status:  MEDLINE    
The subcortical white matter of the adult human brain harbors a pool of glial progenitor cells. These cells can be isolated by fluorescence-activated cell sorting (FACS) after either transfection with green fluorescent protein (GFP) under the control of the CNP2 promoter, or A2B5-targeted immunotagging. Although these cells give rise largely to oligodendrocytes, in low-density culture we observed that some also generated neurons. We thus asked whether these nominally glial progenitors might include multipotential progenitor cells capable of neurogenesis. We found that adult human white-matter progenitor cells (WMPCs) could be passaged as neurospheres in vitro and that these cells generated functionally competent neurons and glia both in vitro and after xenograft to the fetal rat brain. WMPCs were able to produce neurons after their initial isolation and did not require in vitro expansion or reprogramming to do so. These experiments indicate that an abundant pool of mitotically competent neurogenic progenitor cells resides in the adult human white matter.
Marta C Nunes; Neeta Singh Roy; H Michael Keyoung; Robert R Goodman; Guy McKhann; Li Jiang; Jian Kang; Maiken Nedergaard; Steven A Goldman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-03-10
Journal Detail:
Title:  Nature medicine     Volume:  9     ISSN:  1078-8956     ISO Abbreviation:  Nat. Med.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-03     Completed Date:  2003-05-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9502015     Medline TA:  Nat Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  439-47     Citation Subset:  IM    
Department of Neurology and Neuroscience, Cornell University Medical College, New York, New York, USA.
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MeSH Terms
Brain / cytology*,  embryology
Brain Tissue Transplantation
Cell Differentiation
Cell Division
Cell Separation
Cells, Cultured
Child, Preschool
Flow Cytometry
Middle Aged
Neuroglia / cytology,  physiology*,  transplantation
Neurons / cytology,  physiology*,  transplantation
Stem Cell Transplantation
Stem Cells / cytology,  physiology*
Grant Support

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