| Identification and isolation of lantibiotics from culture: a bioorthogonal chemistry approach. | |
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MedLine Citation:
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PMID: 23033043 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A distinguishing feature of the lantibiotic family of cyclic peptides is the presence of thioethers. Treatment of a lantibiotic with an alkaline solution at high pH gives rise to a β-elimination reaction yielding the corresponding ring opened precursor, containing a dehydro-amino acid residue. We here reveal in a proof-of-concept study that a ring opened lantibiotic (mersacidin) can be captured for pull-down from a culture broth, subsequently released and identified by mass spectrometry. |
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Authors:
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Jing Li; Genevieve Girard; Bogdan I Florea; Paul P Geurink; Nan Li; Gijsbert A van der Marel; Mark Overhand; Herman S Overkleeft; Gilles P van Wezel |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
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Title: Organic & biomolecular chemistry Volume: - ISSN: 1477-0539 ISO Abbreviation: Org. Biomol. Chem. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101154995 Medline TA: Org Biomol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Leiden Institute of Chemistry and Netherlands Proteomics Centre, Gorlaeus Laboratories, Einsteinweg 55, 2333 CC Leiden, The Netherlands. h.s.overkleeft@chem.leidenuniv.nl. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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