| Identification of isobaric amino-sulfonamides without prior separation. | |
| | |
MedLine Citation:
|
PMID: 22279018 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
RATIONALE: Direct analysis mass spectrometry (DAMS) techniques offer increased speed of analysis without the need for sample preparation or prior separation. A feature of these techniques is that all ionisable species will typically be analysed at the same time which makes the ability to distinguish between isobaric compounds increasingly important. METHODS: Investigations have been carried out to distinguish isomeric compounds by mass spectrometry only, without the use of any separation technique, in order to further understand the capabilities of DAMS techniques. The work focused on commercially available isomeric amino-sulfonamides, i.e. sulfalene, sulfameter, sulfamethoxypyridazine, sulfamonomethoxine, sulfadoxine, sulfadimethoxine, sulfisomidine, sulfamethazine, sulfamerazine, sulfaperine, sulfadiazine and sulfapyrazine. RESULTS: All the isomeric compounds investigated could be distinguished from each other based on their tandem mass (MS/MS) spectrum or failing that, based on their MS(3) spectrum. Common fragmentation patterns/pathways were observed for groups of the sulfonamides and a rationale for the fragmentations observed is proposed. For the sulfonamides which contain a methoxy group on the pyrimidinyl, pyridazynil, or pyrazinyl ring, the fragmentation-directing feature is the positioning of the methoxy group in the ortho position of the ring with respect to the sulfonamide bond. The presence of an ortho substituent precludes the formation of the product ion resulting from the loss of aniline. CONCLUSIONS: This work has demonstrated the usefulness of MS(n) fragmentation data in identifying and distinguishing isobaric structural isomers without the need for separation by high-performance liquid chromatography (HPLC), allowing the identification of compounds by DAMS techniques. This work has also highlighted patterns in the product ion data which has led to a postulation of how the protonation preference of a molecule can affect the product ions observed and how the presence of ortho substituents can affect this initial protonation preference. Copyright © 2012 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Samantha J Barry; Jean-Claude Wolff |
Related Documents
:
|
22265518 - Determination of nicotine exposure in dogs subjected to passive smoking using methanol ... 1669578 - Semiquantitative determination of serum ige by reagent strips: prist/totale correlation. 22533568 - Recent advances in metabolite identification and quantitative bioanalysis by lc-q-tof ms. 22678558 - Structural characterisation and identification of phenylethanoid glycosides from cistan... 20061238 - Urine ochratoxin a and sphinganine/sphingosine ratio in residents of the endemic nephro... 3782348 - Operational parameters of voltammetric high-performance liquid chromatographic detector... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Rapid communications in mass spectrometry : RCM Volume: 26 ISSN: 1097-0231 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-01-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
|
Languages: eng Pagination: 419-29 Citation Subset: IM |
Copyright Information:
|
Copyright © 2012 John Wiley & Sons, Ltd. |
Affiliation:
|
GlaxoSmithKline, Analytical Sciences, Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK. samantha.j.barry@gsk.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Direct detection of additives and degradation products from polymers by liquid extraction surface an...
Next Document: The effect of (15)N to (14)N ratio on nitrification, denitrification and dissimilatory nitrate reduc...