Document Detail

Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase.
MedLine Citation:
PMID:  16101307     Owner:  NLM     Status:  MEDLINE    
A d-galacturonic acid reductase and the corresponding gene were identified from the mold Hypocrea jecorina (Trichoderma reesei). We hypothesize that the enzyme is part of a fungal d-galacturonic acid catabolic pathway which has not been described previously and which is distinctly different from the bacterial pathway. H. jecorina grown on d-galacturonic acid exhibits an NADPH-dependent d-galacturonic acid reductase activity. This activity is absent when the mold is grown on other carbon sources. The d-galacturonic acid reductase was purified, and tryptic digests of the purified protein were sequenced. The open reading frame of the corresponding gene was then cloned from a cDNA library. The open reading frame was functionally expressed in the yeast Saccharomyces cerevisiae. A histidine-tagged protein was purified, and the enzyme kinetics were characterized. The enzyme converts in a reversible reaction from d-galacturonic acid and NADPH to l-galactonic acid and NADP. The enzyme also exhibits activity with d-glucuronic acid and dl-glyceraldehyde.
Satu Kuorelahti; Nisse Kalkkinen; Merja Penttilä; John Londesborough; Peter Richard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  44     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-16     Completed Date:  2005-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11234-40     Citation Subset:  IM    
VTT Biotechnology, P.O. Box 1500, 02044 VTT, Espoo, Finland.
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MeSH Terms
Alcohol Oxidoreductases / chemistry*,  genetics,  metabolism
Base Sequence
Cloning, Molecular
Fungal Proteins / chemistry*,  genetics,  metabolism
Gene Expression
Gene Library
Hexuronic Acids / chemistry*,  metabolism
Hypocrea / enzymology*,  genetics
Molecular Sequence Data
NADP / chemistry,  metabolism
Saccharomyces cerevisiae / genetics
Substrate Specificity / physiology
Sugar Acids / chemistry,  metabolism
Reg. No./Substance:
0/Fungal Proteins; 0/Hexuronic Acids; 0/Sugar Acids; 13382-27-9/galactonic acid; 14982-50-4/galacturonic acid; 53-59-8/NADP; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.1.-/D-galacturonic acid reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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