Document Detail


Identification of human glandular kallikrein in the beta cell of the pancreas.
MedLine Citation:
PMID:  220534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the cellular localization of glandular kallikrein in the human pancreas, immunohistochemical studies were performed with a monospecific antibody against the antigenically identical urinary kallikrein (urokallikrein). The localization of glandular pancreatic kallikrein to the beta cells of the islets was the same as that of insulin in normal human pancreas and in two islet-cell tumors. When beta cells were lacking in islet-cell tumors or in the pancreas of a patient with juvenile-onset diabetes, kallikrein antigen was not detectable. Anti-urokallikrein absorbed with purified urinary or pancreatic kallikrein no longer identified a pancreatic antigen, whereas absorption with insulin had no effect. The beta-cell localization of human pancreatic kallikrein, an endopeptidase that, in concert with carboxypeptidase B, converts bovine proinsulin to a polypeptide with the electrophoretic mobility of insulin, suggests that pancreatic kallikrein may be involved in the physiologic activation of proinsulin.
Authors:
O K ole-MoiYoi; G S Pinkus; J Spragg; K F Austen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The New England journal of medicine     Volume:  300     ISSN:  0028-4793     ISO Abbreviation:  N. Engl. J. Med.     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-07-16     Completed Date:  1979-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0255562     Medline TA:  N Engl J Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1289-94     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adenoma, Islet Cell / chemistry
Antibodies / analysis
Antigens / analysis
Diabetes Mellitus, Type 1 / enzymology
Electrophoresis, Polyacrylamide Gel
Humans
Immunodiffusion
Immunologic Techniques
Insulin / isolation & purification
Islets of Langerhans / chemistry*,  enzymology,  immunology
Kallikreins / immunology,  isolation & purification*,  physiology,  urine
Proinsulin / isolation & purification
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens; 11061-68-0/Insulin; 9035-68-1/Proinsulin; EC 3.4.21.-/Kallikreins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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