| Identification of human germinal center light and dark zone cells and their relationship to human B-cell lymphomas. | |
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MedLine Citation:
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PMID: 22740445 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Germinal centers (GCs) are sites of B-cell clonal expansion, hypermutation, and selection. GCs are polarized into dark (DZ) and light zones (LZ), a distinction that is of key importance to GC selection. However, the difference between the B cells in each of these zones in humans remains unclear. We show that, as in mice, CXCR4 and CD83 can be used to distinguish human LZ and DZ cells. Using these markers, we show that LZ and DZ cells in mice and humans differ only in the expression of characteristic "activation" and "proliferation" programs, suggesting that these populations represent alternating states of a single-cell type rather than distinct differentiation stages. In addition, LZ/DZ transcriptional profiling shows that, with the exception of "molecular" Burkitt lymphomas, nearly all human B-cell malignancies closely resemble LZ cells, which has important implications for our understanding of the molecular programs of lymphomagenesis. |
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Authors:
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Gabriel D Victora; David Dominguez-Sola; Antony B Holmes; Stephanie Deroubaix; Riccardo Dalla-Favera; Michel C Nussenzweig |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-06-26 |
Journal Detail:
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Title: Blood Volume: 120 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-14 Completed Date: 2012-12-03 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 2240-8 Citation Subset: AIM; IM |
Affiliation:
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Laboratory of Molecular Immunology, Rockefeller University, New York, NY, USA. victora@wi.mit.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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GEO/GSE38696; GSE38697 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / genetics, metabolism Burkitt Lymphoma / immunology, metabolism, pathology Cells, Cultured Child Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Germinal Center / immunology, metabolism, pathology* Humans Immunoglobulins / genetics, metabolism Leukocytes, Mononuclear / cytology, immunology, metabolism, pathology Lymph Nodes / cytology, immunology, metabolism Lymphoma, B-Cell / immunology, metabolism, pathology* Male Membrane Glycoproteins / genetics, metabolism Mice Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis Palatine Tonsil / immunology, metabolism, pathology Receptors, CXCR4 / genetics, metabolism Species Specificity |
| Grant Support | |
ID/Acronym/Agency:
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K99/R00 CA151827/CA/NCI NIH HHS; R01 AI037526/AI/NIAID NIH HHS; R01 AI072529/AI/NIAID NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/CD83 antigen; 0/Immunoglobulins; 0/Membrane Glycoproteins; 0/Receptors, CXCR4 |
| Comments/Corrections | |
Comment In:
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Blood. 2012 Sep 13;120(11):2158-9
[PMID:
22977079
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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