Document Detail

Identification of human germinal center light and dark zone cells and their relationship to human B-cell lymphomas.
MedLine Citation:
PMID:  22740445     Owner:  NLM     Status:  MEDLINE    
Germinal centers (GCs) are sites of B-cell clonal expansion, hypermutation, and selection. GCs are polarized into dark (DZ) and light zones (LZ), a distinction that is of key importance to GC selection. However, the difference between the B cells in each of these zones in humans remains unclear. We show that, as in mice, CXCR4 and CD83 can be used to distinguish human LZ and DZ cells. Using these markers, we show that LZ and DZ cells in mice and humans differ only in the expression of characteristic "activation" and "proliferation" programs, suggesting that these populations represent alternating states of a single-cell type rather than distinct differentiation stages. In addition, LZ/DZ transcriptional profiling shows that, with the exception of "molecular" Burkitt lymphomas, nearly all human B-cell malignancies closely resemble LZ cells, which has important implications for our understanding of the molecular programs of lymphomagenesis.
Gabriel D Victora; David Dominguez-Sola; Antony B Holmes; Stephanie Deroubaix; Riccardo Dalla-Favera; Michel C Nussenzweig
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-26
Journal Detail:
Title:  Blood     Volume:  120     ISSN:  1528-0020     ISO Abbreviation:  Blood     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-14     Completed Date:  2012-12-03     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2240-8     Citation Subset:  AIM; IM    
Laboratory of Molecular Immunology, Rockefeller University, New York, NY, USA.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE38696;  GSE38697
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MeSH Terms
Antigens, CD / genetics,  metabolism
Burkitt Lymphoma / immunology,  metabolism,  pathology
Cells, Cultured
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Germinal Center / immunology,  metabolism,  pathology*
Immunoglobulins / genetics,  metabolism
Leukocytes, Mononuclear / cytology,  immunology,  metabolism,  pathology
Lymph Nodes / cytology,  immunology,  metabolism
Lymphoma, B-Cell / immunology,  metabolism,  pathology*
Membrane Glycoproteins / genetics,  metabolism
Mice, Inbred C57BL
Oligonucleotide Array Sequence Analysis
Palatine Tonsil / immunology,  metabolism,  pathology
Receptors, CXCR4 / genetics,  metabolism
Species Specificity
Grant Support
K99/R00 CA151827/CA/NCI NIH HHS; R01 AI037526/AI/NIAID NIH HHS; R01 AI072529/AI/NIAID NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Antigens, CD; 0/CD83 antigen; 0/Immunoglobulins; 0/Membrane Glycoproteins; 0/Receptors, CXCR4
Comment In:
Blood. 2012 Sep 13;120(11):2158-9   [PMID:  22977079 ]

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