Document Detail


Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family.
MedLine Citation:
PMID:  19137300     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between het-c and het-d, het-c and het-e, het-r and het-v. het-d and het-e are paralogues belonging to the HNWD gene family that encode proteins of the STAND class. HET-D and HET-E proteins comprise an N-terminal HET effector domain, a central GTP binding site and a C-terminal WD repeat domain constituted of tandem repeats of highly conserved WD40 repeat units that define the specificity of alleles during incompatibility. The WD40 repeat units of the members of this HNWD family are undergoing concerted evolution. By combining genetic analysis and gain of function experiments, we demonstrate that an additional member of this family, HNWD2, corresponds to the het-r non-allelic incompatibility gene. As for het-d and het-e, allele specificity at the het-r locus is determined by the WD repeat domain. Natural isolates show allelic variation for het-r.
Authors:
Damien Chevanne; Eric Bastiaans; Alfons Debets; Sven J Saupe; Corinne Clavé; Mathieu Paoletti
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-10
Journal Detail:
Title:  Current genetics     Volume:  55     ISSN:  1432-0983     ISO Abbreviation:  Curr. Genet.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  93-102     Citation Subset:  IM    
Affiliation:
Laboratoire de Génétique Moléculaire des Champignons, IBGC, UMR5095, Université Victor Segalen Bordeaux2 et CNRS, 1 rue Camille Saint-Saëns, 33077, Bordeaux Cedex, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FJ269239;  FJ269240
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MeSH Terms
Descriptor/Qualifier:
Alleles
Cloning, Molecular
DNA, Fungal / genetics,  metabolism
Fungal Proteins / genetics*,  metabolism
Genetic Complementation Test
Linkage (Genetics)
Molecular Sequence Data
Phenotype
Phylogeny
Podospora / genetics*,  metabolism
Polymorphism, Genetic
Repetitive Sequences, Amino Acid / physiology*
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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