Document Detail


Identification of hepatocytic and bile ductular cell lineages and candidate stem cells in bipolar ductular reactions in cirrhotic human liver.
MedLine Citation:
PMID:  17326146     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hepatocyte function and regeneration are severely compromised in severe liver disease, and a common sequela is cirrhosis. Structural changes caused by cirrhosis create a cellular environment conducive to the formation of ductular reactions (DRs). Ductular reactions are primarily composed of oval cells also known as "intermediate hepatobiliary cells". We have conducted single, double, and triple staining to study lineages of oval cells present in DRs. Staining with NCAM, CK19, and HepPar1 has revealed a distinctly bipolar structure to DRs that are embedded in cirrhotic tissue. Spatial analysis of cells that are singly HepPar1-positive, or CK19-positive, has revealed hepatocytic and biliary poles, respectively, in the DRs. Also, the location of singly NCAM-positive cells in DRs suggests that they may be bipotent liver stem/progenitor cells. The locations of other intermediate hepatobiliary cells, which have combinations of markers, suggest that CK19+/NCAM+ cells are transitional cells in the biliary lineage and that rare cells that are negative for all three markers are transitional cells in the hepatocytic lineage. A working cell lineage model for DRs is presented.
Authors:
Hongchao Zhou; Leslie E Rogler; Lewis Teperman; Glyn Morgan; Charles E Rogler
Related Documents :
1238166 - Unscheduled dna synthesis in cells from n-2-fluorenylacetamide-induced hyperplastic nod...
2827496 - Demonstration of glucose-6-phosphatase and peroxisomal catalase activity by ultrastruct...
3031916 - Topogenesis of glycogen distribution pattern in post-natal rat liver in reference to th...
16758626 - The possibility of cytochrome p450 1a1/1a2 induction in cells of distant lymph nodes of...
1447036 - Enhanced induction of tissue-type plasminogen activator in normal human cells compared ...
15639336 - 4'-o-alkyaloenin derivatives and their sulfates directed toward overcoming multidrug re...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  45     ISSN:  0270-9139     ISO Abbreviation:  Hepatology     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-05     Completed Date:  2007-03-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  716-24     Citation Subset:  IM    
Affiliation:
Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies / metabolism
Bile Ducts / metabolism,  pathology*
Cell Lineage*
Hepatocytes / metabolism,  pathology*
Humans
Immunohistochemistry
Liver Cirrhosis / metabolism,  pathology*
Liver Regeneration / physiology
Neural Cell Adhesion Molecules / immunology,  metabolism
Proteins / immunology,  metabolism
Stem Cells / metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Neural Cell Adhesion Molecules; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Revelation of simple and complex liver acini after portal transplantation of pancreatic islets or th...
Next Document:  Diagnostic value of HSP70, glypican 3, and glutamine synthetase in hepatocellular nodules in cirrhos...