| Identification of hepatic peroxisomal phospholipase A(2) and characterization of arachidonic acid-containing choline glycerophospholipids in hepatic peroxisomes. | |
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MedLine Citation:
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PMID: 12832049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, a sequence encoding a novel mammalian calcium-independent phospholipase A(2) (iPLA(2)gamma) was identified in the human genome and subsequently cloned and expressed in Sf9 insect cells. Unexpectedly, expression studies in recombinant systems demonstrated the usage of multiple translation initiation codons resulting in different polypeptides. Herein, we demonstrate that hepatic iPLA(2)gamma is localized to rat liver peroxisomes, possesses a molecular mass of 63 kDa and that peroxisomal membranes are highly enriched in arachidonic acid-containing phospholipids. Collectively, these results provide the first demonstration of iPLA(2)gamma in mammalian tissue and suggest the possibility that iPLA(2)gamma can contribute to lipid second messenger generation by hydrolysis of peroxisomal arachidonic acid-containing phospholipids. |
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Authors:
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Jingyue Yang; Xianlin Han; Richard W Gross |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 546 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-30 Completed Date: 2003-08-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 247-50 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Washington University, Campus Box 1134, One Brookings Drive, St Louis, MO 63130, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / metabolism* Blotting, Western Humans Liver / enzymology* Peroxisomes / enzymology* Phosphatidylcholines / metabolism* Phospholipases A / metabolism* Second Messenger Systems Spectrometry, Mass, Electrospray Ionization |
| Grant Support | |
ID/Acronym/Agency:
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P01 HL57278-06A1/HL/NHLBI NIH HHS; P30 DK -56341/DK/NIDDK NIH HHS; P41 RR-00954/RR/NCRR NIH HHS; P60 DK-20579/DK/NIDDK NIH HHS; R01 HL42150-10/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 506-32-1/Arachidonic Acid; EC 3.1.1.-/Phospholipases A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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