Document Detail

Identification of a group of Mus dunni endogenous virus-like endogenous retroviruses from the C57BL/6J mouse genome: proviral genomes, strain distribution, expression characteristics, and genomic integration profile.
MedLine Citation:
PMID:  23197326     Owner:  NLM     Status:  MEDLINE    
About 10 % of the mouse genome is occupied by sequences associated with endogenous retroviruses (ERVs). However, a comprehensive profile of the mouse ERVs and related elements has not been established yet. In this study, we identified a group of ERVs from the mouse genome and characterized their biological properties. Using a custom ERV mining protocol, 191 ERVs (159 loci reported previously and 32 new loci), tentatively named Mus dunni endogenous virus (MDEV)-like ERVs (MDL-ERVs), were mapped on the C57BL/6J mouse genome. Seven of them retained putative full coding potentials for three retroviral polypeptides (gag, pol, and env). Among the 57 mouse strains examined, all but the Mus pahari/Ei strain had PCR amplicons corresponding to a conserved MDL-ERV region. Interestingly, the Mus caroli/EiJ's amplicon was somewhat larger than the others, coinciding with a substantial phylogenetic distance between the MDL-ERV populations of M. caroli/EiJ and C57BL/6J strains. MDL-ERVs were highly expressed in the lung, spleen, and thymus of C57BL/6J mice compared to the brain, heart, kidney, and liver. Seven MDL-ERVs were mapped in the introns of six annotated genes. Of interest, some MDL-ERVs were mapped periodically on three clusters in chromosome X. The finding that these MDL-ERVs were one of several types of retroelements, which form mosaic-repeat units of tandem arrays, suggests that the formation of the mosaic-repeat unit preceded the tandem arrangement event. Further studies are warranted to understand the biological roles of MDL-ERVs in both normal and pathologic conditions.
Kang-Hoon Lee; Ri-Na You; David G Greenhalgh; Kiho Cho
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-30
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  20     ISSN:  1573-6849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-18     Completed Date:  2013-05-20     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  859-74     Citation Subset:  IM    
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MeSH Terms
Brain / virology
Chromosome Mapping*
Cloning, Molecular
Endogenous Retroviruses / classification,  genetics*,  isolation & purification*
Gene Expression Profiling
Genes, Reporter
Genes, env
Genes, gag
Genes, pol
Genetic Loci
Genome / genetics*
Heart / virology
Kidney / virology
Liver / virology
Lung / virology
Mice, Inbred C57BL
Promoter Regions, Genetic
RNA / genetics,  isolation & purification
Sequence Analysis, DNA
Spleen / virology
Thymus Gland / virology
Transcription, Genetic
Grant Support
Reg. No./Substance:

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