| Identification of glycation at the N-terminus of albumin by gas chromatography-mass spectrometry. | |
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MedLine Citation:
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PMID: 2803249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Amino groups in human albumin are modified in vivo by glucose in a non-enzymic reaction, and previous studies have implicated lysine residues as exclusive participants. An investigation using g.c.-m.s. was undertaken to ascertain whether or not the N-terminus was also involved. Appropriate model compounds [N-(1-deoxyglucitol-1-yl) and N-(1-deoxymannitol-1-yl) adducts of aspartic acid] were synthesized and the diagnostic fragment ions of suitable derivatives were established under electron-impact and negative-chemical-ionization conditions. Characteristic fragment ions were identical with those obtained from the model compounds in the mass spectra of derivatives prepared from hydrolysates of reduced albumin. A purified mixture of the model compounds was also obtained from such hydrolysates. Use of radioisotopic incorporation demonstrated that the relative extent of glycation of the epsilon-amino and alpha-amino groups in albumin was approx. 8:1. N-1-Deoxyhexitol adducts of aspartic acid were also identified in reduced and hydrolysed peptides of human urine. |
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Authors:
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D A Robb; O S Olufemi; D A Williams; J M Midgley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 261 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1989 Aug |
Date Detail:
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Created Date: 1989-11-09 Completed Date: 1989-11-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 871-8 Citation Subset: IM |
Affiliation:
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Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, Scotland, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Albumins* Gas Chromatography-Mass Spectrometry Glucose Glycosylation Humans |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 50-99-7/Glucose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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