Document Detail

Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG-12.
MedLine Citation:
PMID:  17440958     Owner:  NLM     Status:  MEDLINE    
The purpose of the present study is to establish and characterize a conditionally immortalized astrocyte cell line and to clarify the genetic networks responsible for the cell growth arrest and differentiation. A conditionally immortalized astrocyte cell line, RCG-12, was established by infecting primary cultured rat cortical glia cells with a temperature-sensitive simian virus 40 large T-antigen. At a permissive temperature of 33 degrees C, the large T-antigen was expressed and cells grew continuously. On the other hand, the down-regulation of T-antigen at a non-permissive temperature of 39 degrees C led to growth arrest and differentiation. The cells expressed astrocyte-expressed genes such as glial fibrillary acidic protein. Interestingly, the differentiated condition induced by the non-permissive temperature significantly elevated the expression levels of several astrocyte-expressed genes. To identify the detailed mechanisms by which non-permissive temperature-induced cell growth arrest and differentiation, we performed high-density oligonucleotide microarray analysis and found that 556 out of 15,923 probe sets were differentially expressed 2.0-fold. A computational gene network analysis revealed that a genetic network containing up-regulated genes such as RB, NOTCH1, and CDKN1A was associated with the cellular growth and proliferation, and that a genetic network containing down-regulated genes such as MYC, CCNB1, and IGF1 was associated with the cell cycle. The established cell line RCG-12 retains some characteristics of astrocytes and should provide an excellent model for studies of astrocyte biology. The present results will also provide a basis for understanding the detailed molecular mechanisms of the growth arrest and differentiation of astrocytes.
Ichiro Takasaki; Satoko Takarada; Mamoru Fukuchi; Makoto Yasuda; Masaaki Tsuda; Yoshiaki Tabuchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  102     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-26     Completed Date:  2008-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1472-85     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 Wiley-Liss, Inc.
Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.
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MeSH Terms
Antigens, Polyomavirus Transforming / metabolism
Astrocytes / cytology,  metabolism*,  physiology*
Cell Count
Cell Differentiation*
Cell Line, Transformed
Cell Proliferation
Cell Transformation, Viral
Cerebral Cortex / cytology
Computational Biology / methods
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Embryo, Mammalian
Gene Expression Regulation / physiology*
Gene Regulatory Networks*
Glial Fibrillary Acidic Protein / metabolism
Models, Genetic
Oligonucleotide Array Sequence Analysis
Rats, Sprague-Dawley
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Cdkn1a protein, rat; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Glial Fibrillary Acidic Protein

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