Document Detail

Identification of genes expressed in the mouse limb using a novel ZPA microarray approach.
MedLine Citation:
PMID:  17911046     Owner:  NLM     Status:  MEDLINE    
One well-studied signaling center in the developing vertebrate limb, the zone of polarizing activity (ZPA), produces the morphogen sonic hedgehog that is necessary for normal growth and pattern formation. To identify additional factors expressed in the ZPA of the mouse limb bud, the Shhgfpcre allele was used to purify ZPA cells using fluorescence-activated cell sorting. Microarray technology was then used to identify genes whose expression was elevated in the ZPA compared to the rest of the limb. In situ hybridization confirmed the expression of two known transcription factors, Hlxb9 and Tcfap2b, an uncharacterized EST, and a transmembrane protein of unknown function in domains overlapping the ZPA. The expression of two other genes was confirmed by rtPCR. The methods described in this report will be applicable for identifying genes enriched in Shh-expressing cells throughout development.
Jason R Rock; M Cecilia Lopez; Henry V Baker; Brian D Harfe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-02
Journal Detail:
Title:  Gene expression patterns : GEP     Volume:  8     ISSN:  1567-133X     ISO Abbreviation:  Gene Expr. Patterns     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-16     Completed Date:  2008-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167473     Medline TA:  Gene Expr Patterns     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  19-26     Citation Subset:  IM    
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Cancer and Genetics Research Complex, 1376 Mowry Road, Gainesville, FL 32610, United States.
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MeSH Terms
Extremities / embryology,  growth & development*
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Hedgehog Proteins / genetics
Homeodomain Proteins / genetics
In Situ Hybridization
Intercellular Signaling Peptides and Proteins / genetics*
Transcription Factor AP-2 / genetics
Transcription Factors / genetics*
Reg. No./Substance:
0/Hedgehog Proteins; 0/Homeodomain Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/SHH protein, human; 0/Tcfap2b protein, mouse; 0/Transcription Factor AP-2; 0/Transcription Factors; 140115-73-7/Hb9 protein, mouse

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