Document Detail

Identification of the genes associated with a virulent strain of Porphyromonas gingivalis using the subtractive hybridization technique.
MedLine Citation:
PMID:  18173803     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Porphyromonas gingivalis, a major etiological organism implicated in periodontal disease, can be classified into virulent and avirulent strains. Our aim was to identify a gene for the virulence of P. gingivalis. METHODS: The subtractive hybridization technique was employed to identify the genes specific to P. gingivalis W83, a virulent strain. In this study, P. gingivalis W83 was used as the tester strain, and P. gingivalis ATCC 33277 was the driver strain. The prevalence of W83-specific genes was determined by Southern blot analysis of several P. gingivalis strains. RESULTS: We obtained 575 colonies using the subtractive hybridization technique. From among these, 26 DNA fragments were subjected to a homology search using the BLAST program. Compared with strain ATCC 33277, strain W83 contained 12 unique clones. The specificities of the isolated DNA fragments were analyzed among four P. gingivalis strains by Southern blot analysis. Five genes showed specificity for strain W83 compared with strain ATCC 33277. All five genes were also identified in strain W50. CONCLUSIONS: The subtractive hybridization technique was effective in screening the two strains for specific DNA sequences, some of which might be responsible for determining virulence. The results suggested that several genes specific to strain W83 were associated with its virulence. Further analysis of these DNA fragments will provide important information on the pathogenesis of virulent P. gingivalis strains.
M Tachibana-Ono; A Yoshida; S Kataoka; T Ansai; Y Shintani; Y Takahashi; K Toyoshima; T Takehara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral microbiology and immunology     Volume:  23     ISSN:  0902-0055     ISO Abbreviation:  Oral Microbiol. Immunol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-04     Completed Date:  2008-04-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8707451     Medline TA:  Oral Microbiol Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  84-7     Citation Subset:  D    
Division of Community Oral Health Science, Kyushu Dental College, Kokurakita-ku, Kitakyushu, Japan.
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MeSH Terms
Bacterial Capsules / genetics
Bacterial Proteins / genetics
Blotting, Southern
DNA Helicases / genetics
DNA, Bacterial / genetics
Fibronectins / genetics
Genes, Bacterial / genetics*
Glycosyltransferases / genetics
Nucleic Acid Hybridization
Polymerase Chain Reaction
Polysaccharides, Bacterial / genetics
Porphyromonas gingivalis / genetics,  pathogenicity*
Sequence Homology, Nucleic Acid
Transposases / genetics
Virulence / genetics*
Reg. No./Substance:
0/Bacterial Capsules; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/Fibronectins; 0/Polysaccharides, Bacterial; EC 2.4.-/Glycosyltransferases; EC 2.4.99.-/murein transglycosylase; EC 2.7.7.-/Transposases; EC 3.6.1.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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