Document Detail

Identification and functional characterization of imatinib-sensitive DTD1-PDGFRB and CCDC88C-PDGFRB fusion genes in eosinophilia-associated myeloid/lymphoid neoplasms.
MedLine Citation:
PMID:  24772479     Owner:  NLM     Status:  In-Process    
Eosinophilia-associated myeloid neoplasms with rearrangement of chromosome bands 5q31-33 are frequently associated with PDGFRB fusion genes, which are exquisitely sensitive to treatment with imatinib. In search for novel fusion partners of PDGFRB, we analyzed three cases with translocation t(5;20)(q33;p11), t(5;14)(q33;q32), and t(5;17;14)(q33;q11;q32) by 5′-rapid amplification of cDNA ends polymerase chain reaction (5′-RACE-PCR) and DNA-based long-distance inverse PCR (LDI-PCR) with primers derived from PDGFRB. LDI-PCR revealed a fusion between CCDC88C exon 25 and PDGFRB exon 11 in the case with t(5;17;14)(q33;q11;q32) while 5′-RACE-PCR identified fusions between CCDC88C exon 10 and PDGFRB exon 12 and between DTD1 exon 4 and PDGFRB exon 12 in the cases with t(5;14)(q33;q32) and t(5;20)(q33;p11), respectively. The PDGFRB tyrosine-kinase domain is predicted to be retained in all three fusion proteins. The partner proteins contained coiled-coil domains or other domains, which putatively lead to constitutive activation of the PDGFRB fusion protein. In vitro functional analyses confirmed transforming activity and imatinib-sensitivity of the fusion proteins. All three patients achieved rapid and durable complete hematologic remissions on imatinib.
Darko Gosenca; Beate Kellert; Georgia Metzgeroth; Claudia Haferlach; Alice Fabarius; Juliana Schwaab; Michael Kneba; Christof Scheid; Karin Töpelt; Philipp Erben; Torsten Haferlach; Nicholas C P Cross; Wolf-Karsten Hofmann; Wolfgang Seifarth; Andreas Reiter
Related Documents :
23560779 - Assembly in vitro of rhodococcus jostii rha1 encapsulin and peroxidase dypb to form ...
24400649 - Experimental design approach in recombinant protein expression: determining medium comp...
25056309 - New insights into the incorporation of natural suppressor trnas at stop codons in sacch...
24129839 - Codon optimization enhances protein expression of bombyx mori nucleopolyhedrovirus dna ...
24980909 - Experimental and bioinformatic characterization of a recombinant polygalacturonase-inhi...
23922919 - Yeast one-hybrid gγ recruitment system for identification of protein lipidation motifs.
21343359 - The cellular protein la functions in enhancement of virus release through lipid rafts f...
8698489 - Identification and characterization of cs20, a new putative colonization factor of ente...
22669819 - The prdm family: expanding roles in stem cells and development.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  53     ISSN:  1098-2264     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-04-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  411-21     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A critical role of hypocretin deficiency in pregnancy.
Next Document:  Neovascularization is prominent in the chronic inflammatory lesions of Sjögren's syndrome.